ArticleBioengineering (Basel, Switzerland)2018
A Precisely Flow-Controlled Microfluidic System for Enhanced Pre-Osteoblastic Cell Response for Bone Tissue Engineering.
Article in Bioengineering (Basel, Switzerland), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 40 citations in OpenAlex.
- Emerging engineering strategies in bone organoids: From biomimetic scaffolds to dynamic microenvironmental stimulation.Bioactive materials · 2026Review
- Computational Assessment of Oxygen Availability and Shear Stress in Microfluidic Cell Culture Chambers for Optimized Cell Adhesion.Biosensors · 2026Article
- Mechanical Stimulation in Bone-on-Chip Platforms: A Systematic Review of Osteogenic Responses in Medical and Dental Applications.Annals of biomedical engineering · 2026Review
- Advances in Bone-on-a-Chips for In Vitro Modeling of Bone Physiology and Pathology.Biomedicines · 2026Review
- Evaluating Complexity in Orthopedic Tissue-on-a-Chip Systems.Advanced healthcare materials · 2026Review
- Geometry-Guided Osteogenesis in Bone-on-a-Chip Systems Using Triply Periodic Minimal Surface Scaffolds.Advanced healthcare materials · 2026Article
- Flow-Induced Shear Stress Combined with Microtopography Inhibits the Differentiation of Neuro-2a Cells.Micromachines · 2025Article
- Bone-on-a-Chip Systems for Hematological Cancers.Biosensors · 2025Review
- A microfluidic organ-on-a-chip: into the next decade of bone tissue engineering applied in dentistry.Future science OA · 2023Review
- Microfluidic Systems for Neural Cell Studies.Bioengineering (Basel, Switzerland) · 2023Review
- Bone/cartilage organoid on-chip: Construction strategy and application.Bioactive materials · 2023Review
- Effect of Uniaxial Compression Frequency on Osteogenic Cell Responses in Dynamic 3D Cultures.Bioengineering (Basel, Switzerland) · 2023Article
- Article
- A bone-on-a-chip collagen hydrogel-based model using pre-differentiated adipose-derived stem cells for personalized bone tissue engineering.Journal of biomedical materials research. Part A · 2023Article
- Direct 3D printed biocompatible microfluidics: assessment of human mesenchymal stem cell differentiation and cytotoxic drug screening in a dynamic culture system.Journal of nanobiotechnology · 2022Article
- Effects of different intensities of intermittent pneumatic soft-tissue compression on bone defect repair.BMC musculoskeletal disorders · 2022Article
- Article
- The role of mechanobiology on the Schwann cell response: A tissue engineering perspective.Frontiers in cellular neuroscience · 2022Review
- Bone-on-a-chip: microfluidic technologies and microphysiologic models of bone tissue.Advanced functional materials · 2021Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bone tissue engineering provides advanced solutions to overcome the limitations of currently used therapies for bone reconstruction. Dynamic culturing of cell-biomaterial constructs positively affects the cell proliferation and differentiation. In this study, we present a precisely flow-controlled microfluidic system employed for the investigation of bone-forming cell responses cultured on fibrous collagen matrices by applying two flow rates, 30 and 50 μL/min. We characterized the collagen substrates morphologically by means of scanning electron microscopy, investigated their viscoelastic properties, and evaluated the orientation, proliferation and osteogenic differentiation capacity of pre-osteoblastic cells cultured on them. The cells are oriented along the direction of the flow at both rates, in contrast to a random orientation observed under static culture conditions. The proliferation of cells after 7 days in culture was increased at both flow rates, with the flow rate of 50 μL/min indicating a significant increase compared to the static culture. The alkaline phosphatase activity after 7 days increased at both flow rates, with the rate of 30 μL/min indicating a significant enhancement compared to static conditions. Our results demonstrate that precisely flow-controlled microfluidic cell culture provides tunable control of the cell microenvironment that directs cellular activities involved in bone regeneration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.