ArticleJournal of clinical virology : the official publication of the Pan American Society for Clinical Virology2018
Cellular DNA quantification in respiratory samples for the normalization of viral load: a real need?
Article in Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers, 1 of them a synthesis that pooled it.
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The trial behind it
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Who cites it
4 citing papers in PubMed, 1 synthesis or guideline pooled it, 9 citations in OpenAlex.
- Systematic review on the association between respiratory virus real-time PCR cycle threshold values and clinical presentation or outcomes.The Journal of antimicrobial chemotherapy · 2021Pooled it
- Article
- Importance of Adequate qPCR Controls in Infection Control.Diagnostics (Basel, Switzerland) · 2021Article
- Efficacy of Unsupervised Self-Collected Mid-Turbinate FLOQSwabs for the Diagnosis of Coronavirus Disease 2019 (COVID-19).Viruses · 2021Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRespiratory tract infections have an enormous social economic impact, with high incidence of hospitalization and high costs. Adequate specimen collection is the first crucial step for the correct diagnosis of viral respiratory infections.
objectivesThe present retrospective study aimed: i) to verify the cell yield obtained from sampling the nasal respiratory tract using mid-turbinate flocked swabs; ii) to evaluate the normalization of viral load, based on cell number; and iii) to compare the kinetics of viral infection obtained with normalized vs non-normalized viral load. STUDY
designThe number of cells were quantified by real-time PCR in residual extract of nasal swabs tested for respiratory viruses detection and stored at -80 °C in a universal transport medium (UTM™).
resultsA total of 513 virus-positive and 226 virus-negative samples were analyzed. Overall, a median of 4.42 log
conclusionsThe normalization of viral load using cellular load compliments the validation of real-time PCR results in the diagnosis of respiratory viruses but is not strictly needed.
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Registered trials
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