ArticleNucleic acids research2018
Displacement of the transcription factor B reader domain during transcription initiation.
Article in Nucleic acids research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 21 citations in OpenAlex.
- Promoter contacts by TFB and TFEα during archaeal transcription initiation.Transcription · 2026Article
- Genetic Code Expansion: Recent Developments and Emerging Applications.Chemical reviews · 2025Review
- A novel RHH family transcription factor aCcr1 and its viral homologs dictate cell cycle progression in archaea.Nucleic acids research · 2023Article
- Promoter-proximal elongation regulates transcription in archaea.Nature communications · 2021Article
- Direct binding of TFEα opens DNA binding cleft of RNA polymerase.Nature communications · 2020Article
- Archaeal transcription.Transcription · 2020Review
- RNA extension drives a stepwise displacement of an initiation-factor structural module in initial transcription.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Structures and mechanism of transcription initiation by bacterial ECF factors.Nucleic acids research · 2019Article
- The cutting edge of archaeal transcription.Emerging topics in life sciences · 2018Article
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Transcription initiation by archaeal RNA polymerase (RNAP) and eukaryotic RNAP II requires the general transcription factor (TF) B/ IIB. Structural analyses of eukaryotic transcription initiation complexes locate the B-reader domain of TFIIB in close proximity to the active site of RNAP II. Here, we present the first crosslinking mapping data that describe the dynamic transitions of an archaeal TFB to provide evidence for structural rearrangements within the transcription complex during transition from initiation to early elongation phase of transcription. Using a highly specific UV-inducible crosslinking system based on the unnatural amino acid para-benzoyl-phenylalanine allowed us to analyze contacts of the Pyrococcus furiosus TFB B-reader domain with site-specific radiolabeled DNA templates in preinitiation and initially transcribing complexes. Crosslink reactions at different initiation steps demonstrate interactions of TFB with DNA at registers +6 to +14, and reduced contacts at +15, with structural transitions of the B-reader domain detected at register +10. Our data suggest that the B-reader domain of TFB interacts with nascent RNA at register +6 and +8 and it is displaced from the transcribed-strand during the transition from +9 to +10, followed by the collapse of the transcription bubble and release of TFB from register +15 onwards.
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Registered trials
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