Evidence map›Paper›PMID 30100066›Full record

ArticleBiochemical and biophysical research communications2018

Optineurin E50K triggers BDNF deficiency-mediated mitochondrial dysfunction in retinal photoreceptor cell line.

Myoung Sup Shim, Keun-Young Kim, Mark Noh, Ji Yoon Ko, Sangphil Ahn, Michelle A An, Takeshi Iwata, Guy A Perkins, Robert N Weinreb, Won-Kyu Ju

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biochemical and biophysical research communications, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
0.8field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 15 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 2 countries.

Myoung Sup ShimHamilton Glaucoma Center, Shiley Eye Institute and Department of Ophthalmology, University of California San Diego, La Jolla, CA, 92039, USA.
Keun-Young KimNational Center for Microscopy and Imaging Research and Department of Neuroscience, University of California San Diego, La Jolla, CA, 92093, USA.
Mark NohHamilton Glaucoma Center, Shiley Eye Institute and Department of Ophthalmology, University of California San Diego, La Jolla, CA, 92039, USA.
Ji Yoon KoHamilton Glaucoma Center, Shiley Eye Institute and Department of Ophthalmology, University of California San Diego, La Jolla, CA, 92039, USA.
Sangphil AhnHamilton Glaucoma Center, Shiley Eye Institute and Department of Ophthalmology, University of California San Diego, La Jolla, CA, 92039, USA.
Michelle A AnHamilton Glaucoma Center, Shiley Eye Institute and Department of Ophthalmology, University of California San Diego, La Jolla, CA, 92039, USA.
Takeshi IwataDivision of Molecular and Cellular Biology, National Institute of Sensory Organs, National Hospital Organization Tokyo Medical Center, Tokyo, Japan.
Guy A PerkinsNational Center for Microscopy and Imaging Research and Department of Neuroscience, University of California San Diego, La Jolla, CA, 92093, USA.
Robert N WeinrebHamilton Glaucoma Center, Shiley Eye Institute and Department of Ophthalmology, University of California San Diego, La Jolla, CA, 92039, USA.
Won-Kyu JuHamilton Glaucoma Center, Shiley Eye Institute and Department of Ophthalmology, University of California San Diego, La Jolla, CA, 92039, USA. Electronic address: wju@ucsd.edu.
University of California, San Diego · USTokyo Medical Center · JP

Funding

IVEM and Image Analysis ResourceP41GM103412 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI ELLISMAN, MARK H · 2012 to 2019
$17.9M
Vision BiostatisticsP30EY022589 · NEI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI LINDA M ZANGWILL · 2012 to 2026
$10.3M
Mitochondrial Dysfunction in Glaucomatous Optic NeuropathyR01EY018658 · NEI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI JU, WONKYU · 2009 to 2017
$3.5M
NEI NIH HHS P30 EY022589NEI NIH HHS R01 EY018658NIGMS NIH HHS P41 GM103412
6 · The paper itself

Abstract

Optineurin (OPTN) mutations are linked to glaucoma pathology and E50K mutation shows massive cell death in photoreceptor cells and retinal ganglion cells. However, little is known about E50K-mediated mitochondrial dysfunction in photoreceptor cell degeneration. We here show that overexpression of E50K expression triggered BDNF deficiency, leading to Bax activation in RGC-5 cells. BDNF deficiency induced mitochondrial dysfunction by decreasing mitochondrial maximal respiration and reducing intracellular ATP level in RGC-5 cells. However, BDNF deficiency did not alter mitochondrial dynamics. Also, BDNF deficiency resulted in LC3-mediated mitophagosome formation in RGC-5 cells. These results strongly suggest that E50K-mediated BDNF deficiency plays a critical role in compromised mitochondrial function in glaucomatous photoreceptor cell degeneration.

Indexed as

Adenosine TriphosphateAmino Acid SubstitutionAnimalsbcl-2-Associated X ProteinBrain-Derived Neurotrophic FactorCell LineGene Expression RegulationMicrotubule-Associated ProteinsMitochondriaMutationOxidative PhosphorylationPhagosomesPhotoreceptor Cells, VertebrateRatsSignal TransductionTranscription Factor TFIIIAAdenosine TriphosphateAnxa3 protein, ratBax protein, ratbcl-2-Associated X ProteinBdnf protein, ratBrain-Derived Neurotrophic FactorMicrotubule-Associated ProteinsOPTN protein, ratTranscription Factor TFIIIABDNFE50K mutationGlaucomaMitochondrial dysfunctionOptineurinRGC-5 cell

Identifiers

PMID30100066
PMCPMC6133749
OpenAlexW2887321647

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.