Evidence map›Paper›PMID 30099590›Full record

ArticleJournal of molecular evolution2018

Cotranscriptional 3'-End Processing of T7 RNA Polymerase Transcripts by a Smaller HDV Ribozyme.

Arvin Akoopie, Ulrich F Müller

Abstract read
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In one paragraph

Article in Journal of molecular evolution, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.1field-weighted citation impact, top 52% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 3 citations in OpenAlex.

  1. Real-time monitoring strategies for optimization ofFrontiers in molecular biosciences · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Arvin AkoopieDepartment of Chemistry and Biochemistry, University of California San Diego, La Jolla, CA, USA.
Ulrich F MüllerDepartment of Chemistry and Biochemistry, University of California San Diego, La Jolla, CA, USA. ufmuller@ucsd.edu.ORCID 0000-0002-7473-538X
University of California San Diego · US

Funding

NASA NNX13AJ09GU.S. Department of Education GAANN P200A150251
6 · The paper itself

Abstract

In vitro run-off transcription by T7 RNA polymerase generates heterogeneous 3'-ends because the enzyme tends to add untemplated adenylates. To generate homogeneous 3'-termini, HDV ribozymes have been used widely. Their sequences are added to the 3'-terminus such that co-transcriptional self-cleavage generates homogeneous 3'-ends. A shorter HDV sequence that cleaves itself efficiently would be advantageous. Here we show that a recently discovered, small HDV ribozyme is a good alternative to the previously used HDV ribozyme. The new HDV ribozyme is more efficient in some sequence contexts, and less efficient in other sequence contexts than the previously used HDV ribozyme. The smaller size makes the new HDV ribozyme a good alternative for transcript 3'-end processing.

Indexed as

3' Flanking RegionBase SequenceDNA-Directed RNA PolymerasesHepatitis Delta VirusKineticsNucleic Acid ConformationRNA, CatalyticRNA Processing, Post-TranscriptionalRNA, ViralTranscription, GeneticViral Proteinsbacteriophage T7 RNA polymeraseDNA-Directed RNA PolymerasesRNA, CatalyticRNA, ViralViral Proteins3′-TerminusCo-transcriptional processingHDV ribozymeIn vitro transcription

Identifiers

PMID30099590
OpenAlexW2887671677

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.