ArticleMethods (San Diego, Calif.)2019
A guide to nucleic acid detection by single-molecule kinetic fingerprinting.
Article in Methods (San Diego, Calif.), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- FINDER converts zero-background kinetic fingerprinting into area-scalable attomolar biomarker detection.Research square · 2026Article
- FINDER converts zero-background kinetic fingerprinting into area-scalable attomolar biomarker detection.bioRxiv : the preprint server for biology · 2026Article
- RNA detection on a microfluidic platform using Thyclotides.Sensors and actuators reports · 2026Article
- Foundation model for efficient biological discovery in single-molecule time traces.Nature methods · 2025Article
- Chromato-Kinetic Fingerprinting Enables Multiomic Digital Counting of Single Disease Biomarker Molecules.ACS nano · 2025Article
- Ultrasensitive Amplification-Free Quantification of a Methyl CpG-Rich Cancer Biomarker by Single-Molecule Kinetic Fingerprinting.Analytical chemistry · 2024Article
- Mechanistic model for epigenetic maintenance by methyl-CpG-binding domain proteins.bioRxiv : the preprint server for biology · 2024Article
- Highly sensitive protein detection by aptamer-based single-molecule kinetic fingerprinting.Biosensors & bioelectronics · 2022Article
- Attomolar Sensitivity in Single Biomarker Counting upon Aqueous Two-Phase Surface Enrichment.ACS sensors · 2022Article
- A guide to accelerated direct digital counting of single nucleic acid molecules by FRET-based intramolecular kinetic fingerprinting.Methods (San Diego, Calif.) · 2022Review
- Calibration-free counting of low molecular copy numbers in single DNA-PAINT localization clusters.Biophysical reports · 2021Article
- Rapid kinetic fingerprinting of single nucleic acid molecules by a FRET-based dynamic nanosensor.Biosensors & bioelectronics · 2021Article
- A compact photonic resonator absorption microscope for point of care digital resolution nucleic acid molecular diagnostics.Biomedical optics express · 2021Article
- Single-Molecule FRET-Based Dynamic DNA Sensor.ACS sensors · 2021Article
- Direct Kinetic Fingerprinting for High-Accuracy Single-Molecule Counting of Diverse Disease Biomarkers.Accounts of chemical research · 2021Article
- Probing Biosensing Interfaces With Single Molecule Localization Microscopy (SMLM).Frontiers in chemistry · 2021Review
- Automatic classification and segmentation of single-molecule fluorescence time traces with deep learning.Nature communications · 2020Article
- Direct kinetic fingerprinting and digital counting of single protein molecules.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Critical Review: digital resolution biomolecular sensing for diagnostics and life science research.Lab on a chip · 2020Review
- Ultraspecific analyte detection by direct kinetic fingerprinting of single molecules.Trends in analytical chemistry : TRAC · 2020Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Conventional methods for detecting small quantities of nucleic acids require amplification by the polymerase chain reaction (PCR), which necessitates prior purification and introduces copying errors. While amplification-free methods do not have these shortcomings, they are generally orders of magnitude less sensitive and specific than PCR-based methods. In this review, we provide a practical guide to a novel amplification-free method, single-molecule recognition through equilibrium Poisson sampling (SiMREPS), that provides both single-molecule sensitivity and single-base selectivity by monitoring the repetitive interactions of fluorescent probes to immobilized targets. We demonstrate how this kinetic fingerprinting filters out background arising from the inevitable nonspecific binding of probes, yielding virtually zero background signal. As practical applications of this digital detection methodology, we present the quantification of microRNA miR-16 and the detection of the mutation EGFR L858R with an apparent single-base discrimination factor of over 3 million.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.