Evidence map›Paper›PMID 30098521›Full record

ArticleJournal of photochemistry and photobiology. B, Biology2018

Mitochondrial dynamics (fission and fusion) and collagen production in a rat model of diabetic wound healing treated by photobiomodulation: comparison of 904 nm laser and 850 nm light-emitting diode (LED).

José Carlos Tatmatsu-Rocha, Carla Roberta Tim, Lucimar Avo, Rubens Bernardes-Filho, Patricia Brassolatti, Hueliton Wilian Kido, Michael R Hamblin, Nivaldo Antonio Parizotto

Open access · greenAbstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of photochemistry and photobiology. B, Biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed, 2 pooled it
4.7field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed, 2 syntheses or guidelines pooled it, 61 citations in OpenAlex.

  1. Pooled it
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  14. Photobiomodulation in 3D tissue engineering.Journal of biomedical optics · 2022
    Article
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  16. Article
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  19. The Effects of Photobiomodulation on MC3T3-E1 Cells via 630 nm and 810 nm Light-Emitting Diode.Medical science monitor : international medical journal of experimental and clinical research · 2019
    Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 6 institutions in 2 countries.

José Carlos Tatmatsu-RochaMedicine School, Physiotherapy Department, Federal University of Ceara, Fortaleza, Ceara, Brazil. Electronic address: tatmatsu@ufc.br.
Carla Roberta TimBrasil University, Postgraduate program in Biomedical Engineering, Brazil.
Lucimar AvoMedicine Department, Federal University of São Carlos, São Paulo, Brazil.
Rubens Bernardes-FilhoBrazilian Enterprise of Agriculture Research, EMBRAPA, São Carlos, SP, Brazil. Electronic address: rubens.bernardes@embrapa.br.
Patricia BrassolattiBiotechnology Department, Federal University of São Carlos, São Paulo, Brazil.
Hueliton Wilian KidoNove de Julho University (UNINOVE), Bauru, SP, Brazil.
Michael R HamblinWellman Center for Photomedicine, Massachusetts General Hospital, Boston, MA 02114, USA; Department of Dermatology, Harvard Medical School, Boston, MA 02115, USA; Harvard-MIT Division of Health Sciences and Technology, Cambridge, MA 02139, USA.
Nivaldo Antonio ParizottoPhysiotherapy Department, Federal University of Sao Carlos, Sao Paulo, Brazil.
Universidade Federal de São Carlos · BRBrazilian Agricultural Research Corporation · BRHarvard University · USUniversidade Brasil · BRUniversidade Federal do Ceará · BRUniversidade Nove de Julho · BR

Funding

Photodynamic Therapy of Localized InfectionsR01AI050875 · NIAID · MASSACHUSETTS GENERAL HOSPITAL · PI HAMBLIN, MICHAEL R · 2003 to 2017
$4.8M
Synergistic Photodynamic Therapy for Catherer-Associated Urinary Tract InfectionsR21AI121700 · NIAID · MASSACHUSETTS GENERAL HOSPITAL · PI HAMBLIN, MICHAEL R · 2016 to 2017
$462k
NIAID NIH HHS R01 AI050875NIAID NIH HHS R21 AI121700
6 · The paper itself

Abstract

objectiveMitochondrial dysfunction has been associated with the development of diabetes mellitus which is characterized by disorders of collagen production and impaired wound healing. This study analyzed the effects of photobiomodulation (PBM) mediated by laser and light-emitting diode (LED) on the production and organization of collagen fibers in an excisional wound in an animal model of diabetes, and the correlation with inflammation and mitochondrial dynamics.

methodsTwenty Wistar rats were randomized into 4 groups of 5 animals. Groups: (SHAM) a control non-diabetic wounded group with no treatment; (DC) a diabetic wounded group with no treatment; (DLASER) a diabetic wounded group irradiated by 904 nm pulsed laser (40 mW, 9500 Hz, 1 min, 2.4 J); (DLED) a diabetic wounded group irradiated by continuous wave LED 850 nm (48 mW, 22 s, 1.0 J). Diabetes was induced by injection with streptozotocin (70 mg/kg). PBM was carried out daily for 5 days followed by sacrifice and tissue removal.

resultsCollagen fibers in diabetic wounded skin were increased by DLASER but not by DLED. Both groups showed increased blood vessels by atomic force microscopy. Vascular endothelial growth factor (VEGF) was higher and cyclooxygenase (COX2) was lower in the DLED group. Mitochondrial fusion was higher and mitochondrial fusion was lower in DLED compared to DLASER.

conclusionDifferences observed between DLASER and DLED may be due to the pulsed laser and CW LED, and to the higher dose of laser. Regulation of mitochondrial homeostasis may be an important mechanism for PBM effects in diabetes.

Indexed as

LasersLightAnimalsCollagenCyclooxygenase 2Diabetes Mellitus, ExperimentalDisease Models, AnimalGTP PhosphohydrolasesMaleMembrane ProteinsMicroscopy, Atomic ForceMitochondrial DynamicsMitochondrial ProteinsRatsRats, WistarSkinCollagenCyclooxygenase 2GTP PhosphohydrolasesMembrane ProteinsMfn2 protein, ratMitochondrial ProteinsVascular Endothelial Growth Factor AAtomic force microscopyCollagen fibersDiabetes mellitusDiabetic wound healingLaser versus LEDMitochondrial fission and fusionPhotobiomodulation therapy

Identifiers

PMID30098521
PMCPMC6131055
OpenAlexW2886704570

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.