ArticleScientific reports2018
A superfolder variant of pH-sensitive pHluorin for in vivo pH measurements in the endoplasmic reticulum.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 56 citations in OpenAlex.
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- A unified intracellular pH landscape with SITE-pHorin: a quantum-entanglement-enhanced pH probe.Science China. Life sciences · 2026Article
- The pH-dependent photophysical and spectral properties of pH-sensing green fluorescent proteins.Physiological reports · 2025Article
- Arylmethylamino steroid compound 1o interferes withAntimicrobial agents and chemotherapy · 2025Article
- Na + -driven pH regulation by Na+/H+ antiporters promotes photosynthetic efficiency in cyanobacteria.Plant physiology · 2024Article
- ER-plasma membrane contact sites deliver ER lipids and proteins for rapid cell surface expansion.The Journal of cell biology · 2024Article
- Human stem cell-specific epigenetic signatures control transgene expression.Biochimica et biophysica acta. Gene regulatory mechanisms · 2024Article
- Molecular Spies in Action: Genetically Encoded Fluorescent Biosensors Light up Cellular Signals.Chemical reviews · 2024Review
- Osmotic stress induces formation of both liquid condensates and amyloids by a yeast prion domain.The Journal of biological chemistry · 2024Article
- Methods for studying microbial acid stress responses: from molecules to populations.FEMS microbiology reviews · 2024Review
- Real-time measurements of ATP dynamics via ATeams inAntimicrobial agents and chemotherapy · 2024Article
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- A synthetic microbial Daisyworld: planetary regulation in the test tube.Journal of the Royal Society, Interface · 2024Article
- Four ways of implementing robustness quantification in strain characterisation.Biotechnology for biofuels and bioproducts · 2023Article
- The Properties and Domain Requirements for Phase Separation of the Sup35 Prion Protein In Vivo.Biomolecules · 2023Article
- Article
- An optimized reverse β-oxidation pathway to produce selected medium-chain fatty acids in Saccharomyces cerevisiae.Biotechnology for biofuels and bioproducts · 2023Article
- Progress in pH-Sensitive sensors: essential tools for organelle pH detection, spotlighting mitochondrion and diverse applications.Frontiers in pharmacology · 2023Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Many cellular processes are regulated via pH, and maintaining the pH of different organelles is crucial for cell survival. A pH-sensitive GFP variant, the so-called pHluorin, has proven to be a valuable tool to study the pH of the cytosol, mitochondria and other organelles in vivo. We found that the fluorescence intensity of Endoplasmic Reticulum (ER)-targeted pHluorin in the yeast Saccharomyces cerevisiae was very low and barely showed pH sensitivity, probably due to misfolding in the oxidative environment of the ER. We therefore developed a superfolder variant of pHluorin which enabled us to monitor pH changes in the ER and the cytosol of S. cerevisiae in vivo. The superfolder pHluorin variant is likely to be functional in cells of different organisms as well as in additional compartments that originate from the secretory pathway like the Golgi apparatus and pre-vacuolar compartments, and therefore has a broad range of possible future applications.
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