ArticlePLoS genetics2018
Transcription factor ASCL2 is required for development of the glycogen trophoblast cell lineage.
Article in PLoS genetics, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 33 citations in OpenAlex.
- OVOL2 reinforces epithelial identity and promotes SynT-I differentiation in the mouse placenta†.Biology of reproduction · 2026Article
- Loss-of-function mutation of NSD2 is associated with abnormal placentation accompanied by fetal growth retardation in mice.PloS one · 2025Article
- A spatiotemporal transcriptomic atlas of mouse placentation.Cell discovery · 2024Article
- Genomic profiling of selective sweeps through haplotype differentiation unravelled genes associated with production and reproduction traits in Indian goat breeds.Tropical animal health and production · 2024Article
- Fetal growth restriction induced by maternal gal-3 deficiency is associated with altered gut-placenta axis.Cell death & disease · 2024Article
- ASCL2 is a key regulator of the proliferation-differentiation equilibrium in the esophageal epithelium.Biology open · 2024Article
- HIF-1-Induced hsa-miR-429: Understanding Its Direct Targets as the Key to Developing Cancer Diagnostics and Therapies.Cancers · 2023Review
- The multifaceted role of GCM1 during trophoblast differentiation in the human placenta.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Zymosan-A promotes the regeneration of intestinal stem cells by upregulating ASCL2.Cell death & disease · 2022Article
- Article
- Mapping cis-regulatory elements in the midgestation mouse placenta.Scientific reports · 2021Article
- Disabling de novo DNA methylation in embryonic stem cells allows an illegitimate fate trajectory.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- ASCL2 reciprocally controls key trophoblast lineage decisions during hemochorial placenta development.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Regulation of CEACAM5 and Therapeutic Efficacy of an Anti-CEACAM5-SN38 Antibody-drug Conjugate in Neuroendocrine Prostate Cancer.Clinical cancer research : an official journal of the American Association for Cancer Research · 2021Article
- Paternal low protein diet and the supplementation of methyl-donors impact fetal growth and placental development in mice.Placenta · 2021Article
- MicroRNAs from Extracellular Vesicles Secreted by Bovine Embryos as Early Biomarkers of Developmental Competence.International journal of molecular sciences · 2020Article
- TEAD4 ensures postimplantation development by promoting trophoblast self-renewal: An implication in early human pregnancy loss.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Placental imprinting: Emerging mechanisms and functions.PLoS genetics · 2020Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
The basic helix-loop-helix (bHLH) transcription factor ASCL2 plays essential roles in diploid multipotent trophoblast progenitors, intestinal stem cells, follicular T-helper cells, as well as during epidermal development and myogenesis. During early development, Ascl2 expression is regulated by genomic imprinting and only the maternally inherited allele is transcriptionally active in trophoblast. The paternal allele-specific silencing of Ascl2 requires expression of the long non-coding RNA Kcnq1ot1 in cis and the deposition of repressive histone marks. Here we show that Del7AI, a 280-kb deletion allele neighboring Ascl2, interferes with this process in cis and leads to a partial loss of silencing at Ascl2. Genetic rescue experiments show that the low level of Ascl2 expression from the paternal Del7AI allele can rescue the embryonic lethality associated with maternally inherited Ascl2 mutations, in a level-dependent manner. Despite their ability to support development to term, the rescued placentae have a pronounced phenotype characterized by severe hypoplasia of the junctional zone, expansion of the parietal trophoblast giant cell layer, and complete absence of invasive glycogen trophoblast cells. Transcriptome analysis of ectoplacental cones at E7.5 and differentiation assays of Ascl2 mutant trophoblast stem cells show that ASCL2 is required for the emergence or early maintenance of glycogen trophoblast cells during development. Our work identifies a new cis-acting mutation interfering with Kcnq1ot1 silencing function and establishes a novel critical developmental role for the transcription factor ASCL2.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.