ArticleAnalytical chemistry2018
Increased N,N-Dimethyl Leucine Isobaric Tag Multiplexing by a Combined Precursor Isotopic Labeling and Isobaric Tagging Approach.
Article in Analytical chemistry, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- UltraPlex-TMT: Expanding Isobaric Hyperplexing via Orthogonal Protease Cleavage.Journal of proteome research · 2026Article
- A Tutorial Review of Labeling Methods in Mass Spectrometry-Based Quantitative Proteomics.ACS measurement science au · 2024Review
- Establishing Quality Control Procedures for Large-Scale Plasma Proteomics Analyses.Journal of the American Society for Mass Spectrometry · 2023Article
- HyperSCP: Combining Isotopic and Isobaric Labeling for Higher Throughput Single-Cell Proteomics.Analytical chemistry · 2023Article
- Chemical isotope labeling for quantitative proteomics.Mass spectrometry reviews · 2023Review
- Evaluating Linear Ion Trap for MS3-Based Multiplexed Single-Cell Proteomics.Analytical chemistry · 2023Article
- Review
- Mapping the O-GlcNAc Modified Proteome: Applications for Health and Disease.Frontiers in molecular biosciences · 2022Review
- Recent Developments and Applications of Quantitative Proteomics Strategies for High-Throughput Biomolecular Analyses in Cancer Research.RSC chemical biology · 2021Article
- Proteome-wide and matrisome-specific alterations during human pancreas development and maturation.Nature communications · 2021Article
- Recent Advances in Analytical Approaches for Glycan and Glycopeptide Quantitation.Molecular & cellular proteomics : MCP · 2021Review
- Automated Sample Multiplexing by using Combined Precursor Isotopic Labeling and Isobaric Tagging (cPILOT).Journal of visualized experiments : JoVE · 2020Article
- Urinary Amine Metabolomics Characterization with Custom 12-Plex Isobaric DiLeu Labeling.Journal of the American Society for Mass Spectrometry · 2020Article
- 21-plex DiLeu Isobaric Tags for High-Throughput Quantitative Proteomics.Analytical chemistry · 2020Article
- Article
- 27-Plex Tandem Mass Tag Mass Spectrometry for Profiling Brain Proteome in Alzheimer's Disease.Analytical chemistry · 2020Article
- Quantitative proteomics to study aging in rabbit liver.Mechanisms of ageing and development · 2020Article
- Evaluating Combined Precursor Isotopic Labeling and Isobaric Tagging Performance on Orbitraps To Study the Peripheral Proteome of Alzheimer's Disease.Analytical chemistry · 2020Article
- Absolute Quantitation of Oxidizable Peptides by Coulometric Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2019Article
- Nanosecond photochemically promoted click chemistry for enhanced neuropeptide visualization and rapid protein labeling.Nature communications · 2019Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Multiplex isobaric tags have become valuable tools for high-throughput quantitative analysis of complex biological samples in discovery-based proteomics studies. Hybrid labeling strategies that pair stable isotope mass difference labeling with multiplex isobaric tag-based quantification further facilitate these studies by greatly increasing multiplexing capability. In this work, we present a cost-effective chemical labeling approach that couples duplex stable isotope dimethyl labeling with our custom 12-plex N,N-dimethyl leucine (DiLeu) isobaric tags in a combined precursor isotopic labeling and isobaric tagging (cPILOT) strategy that is compatible with a wide variety of biological samples and permits 24-plex quantification in a single LC-MS/MS experiment. We demonstrate the utility of the DiLeu cPILOT approach by labeling yeast digests and performing proof-of-principle quantification experiments on the Orbitrap Fusion Lumos.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.