ArticleScientific reports2018
Stationary and portable sequencing-based approaches for tracing wastewater contamination in urban stormwater systems.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Metagenomic evaluation of bacteria in drinking water using full-length 16S rRNA amplicons.Journal of water and health · 2024Article
- Impacts of an Urban Sanitation Intervention on Fecal Indicators and the Prevalence of Human Fecal Contamination in Mozambique.Environmental science & technology · 2021Article
- Is Oxford Nanopore sequencing ready for analyzing complex microbiomes?FEMS microbiology ecology · 2021Article
- Revealing antimicrobial resistance in stormwater with MinION.Chemosphere · 2020Article
- Metagenomic water quality monitoring with a portable laboratory.Water research · 2020Article
- Microbial Indicators of Fecal Pollution: Recent Progress and Challenges in Assessing Water Quality.Current environmental health reports · 2020Review
- Rapid Detection Methods for Bacterial Pathogens in Ambient Waters at the Point of Sample Collection: A Brief Review.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2020Review
- A lab in the field: applications of real-time,Biology methods & protocols · 2020Article
- Simultaneous detection and comprehensive analysis of HPV and microbiome status of a cervical liquid-based cytology sample using Nanopore MinION sequencing.Scientific reports · 2019Article
- Shotgun metagenome data of a defined mock community using Oxford Nanopore, PacBio and Illumina technologies.Scientific data · 2019Article
- A comparative assessment of conventional and molecular methods, including MinION nanopore sequencing, for surveying water quality.Scientific reports · 2019Article
- Analysing Microbial Community Composition through Amplicon Sequencing: From Sampling to Hypothesis Testing.Frontiers in microbiology · 2017Review
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
Urban sewer systems consist of wastewater and stormwater sewers, of which only wastewater is processed before being discharged. Occasionally, misconnections or damages in the network occur, resulting in untreated wastewater entering natural water bodies via the stormwater system. Cultivation of faecal indicator bacteria (e.g. Escherichia coli; E. coli) is the current standard for tracing wastewater contamination. This method is cheap but has limited specificity and mobility. Here, we compared the E. coli culturing approach with two sequencing-based methodologies (Illumina MiSeq 16S rRNA gene amplicon sequencing and Oxford Nanopore MinION shotgun metagenomic sequencing), analysing 73 stormwater samples collected in Stockholm. High correlations were obtained between E. coli culturing counts and frequencies of human gut microbiome amplicon sequences, indicating E. coli is indeed a good indicator of faecal contamination. However, the amplicon data further holds information on contamination source or alternatively how much time has elapsed since the faecal matter has entered the system. Shotgun metagenomic sequencing on a subset of the samples using a portable real-time sequencer, MinION, correlated well with the amplicon sequencing data. This study demonstrates the use of DNA sequencing to detect human faecal contamination in stormwater systems and the potential of tracing faecal contamination directly in the field.
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