ArticleMolecular metabolism2018
AMPK activation negatively regulates GDAP1, which influences metabolic processes and circadian gene expression in skeletal muscle.
Article in Molecular metabolism, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.
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Who cites it
13 citing papers in PubMed, 1 synthesis or guideline pooled it, 25 citations in OpenAlex.
- Pooled it
- Restoration of circadian rhythm as novel targets against sarcopenia.Chinese medical journal · 2026Review
- Live-cell GLUT4 translocation assay reveals Per3 as a novel regulator of circadian insulin sensitivity in skeletal muscle cells.Biology open · 2025Article
- Ethnicity-related differences in mitochondrial regulation by insulin stimulation in diabetes.Journal of cellular physiology · 2024Review
- Enhancing the metabolic benefits of exercise: Is timing the key?Frontiers in endocrinology · 2023Review
- Time for Exercise? Exercise and Its Influence on the Skeletal Muscle Clock.Journal of biological rhythms · 2022Article
- Time-restricted feeding and cognitive function in sedentary and physically active elderly individuals: Ramadan diurnal intermittent fasting as a model.Frontiers in nutrition · 2022Article
- Exercise as a Peripheral Circadian Clock Resynchronizer in Vascular and Skeletal Muscle Aging.International journal of environmental research and public health · 2021Review
- Disrupted circadian oscillations in type 2 diabetes are linked to altered rhythmic mitochondrial metabolism in skeletal muscle.Science advances · 2021Article
- Inducible deletion of skeletal muscle AMPKα reveals that AMPK is required for nucleotide balance but dispensable for muscle glucose uptake and fat oxidation during exercise.Molecular metabolism · 2020Article
- Ticking for Metabolic Health: The Skeletal-Muscle Clocks.Obesity (Silver Spring, Md.) · 2020Review
- Exercise Timing and Circadian Rhythms.Current opinion in physiology · 2019Article
- Circadian rhythms and exercise - re-setting the clock in metabolic disease.Nature reviews. Endocrinology · 2019Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveWe sought to identify AMPK-regulated genes via bioinformatic analysis of microarray data generated from skeletal muscle of animal models with genetically altered AMPK activity. We hypothesized that such genes would play a role in metabolism. Ganglioside-induced differentiation-associated protein 1 (GDAP1), a gene which plays a role in mitochondrial fission and peroxisomal function in neuronal cells but whose function in skeletal muscle is undescribed, was identified and further validated. AMPK activation reduced GDAP1 expression in skeletal muscle. GDAP1 expression was elevated in skeletal muscle from type 2 diabetic patients but decreased after acute exercise.
methodsThe metabolic impact of GDAP1 silencing was determined in primary skeletal muscle cells via siRNA-transfections. Confocal microscopy was used to visualize whether silencing GDAP1 impacted mitochondrial network morphology and membrane potential.
resultsGDAP1 silencing increased mitochondrial protein abundance, decreased palmitate oxidation, and decreased non-mitochondrial respiration. Mitochondrial morphology was unaltered by GDAP1 silencing. GDAP1 silencing and treatment of cells with AMPK agonists altered several genes in the core molecular clock machinery.
conclusionWe describe a role for GDAP1 in regulating mitochondrial proteins, circadian genes, and metabolic flux in skeletal muscle. Collectively, our results implicate GDAP1 in the circadian control of metabolism.
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