ArticlePloS one2018
High glucose-induced oxidative stress impairs proliferation and migration of human gingival fibroblasts.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 95 papers, 1 of them a synthesis that pooled it.
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Who cites it
95 citing papers in PubMed, 1 synthesis or guideline pooled it, 180 citations in OpenAlex.
- Efficacy of different protocols of non-surgical periodontal therapy in patients with type 2 diabetes: A systematic review and meta-analysis.Journal of periodontal research · 2025Pooled it
- Effects of Vitamin and Amino Acid-Enriched Hyaluronic Acid Gel on the Healing of Oral Mucosa: In Vivo and In Vitro Study.Medicina (Kaunas, Lithuania) · 2021Trial
- Ferulic Acid Modulates High Glucose-Induced MMP-1/TIMP-1 Imbalance and Enhances Wound Closure of Human Gingival Fibroblasts.Biomedicines · 2026Article
- Enzymatically triggered glucose-releasing scaffolds as nutritional supports for millimeter-scale tissue engineering.Materials today. Bio · 2026Article
- Modulating Oxidative Stress and Inflammatory Responses ofGels (Basel, Switzerland) · 2026Article
- Lawsone modulates oxidative stress, apoptosis, and enhances growth factor expression in human dermal fibroblasts under hyperglycemic conditions.Molecular biology reports · 2026Article
- Characterization of nano α-TCP: Setting time, antibacterial activity and cytotoxicity on fibroblast cells.Journal of Taibah University Medical Sciences · 2026Article
- Unveiling the therapeutic role of 3D-cultured mesenchymal stem cells in diabetic foot ulcers through transcriptomic integration and fibroblast modulation.Molecular biology reports · 2026Article
- Photobiomodulation at 830 nm with 5 J/cmLasers in medical science · 2026Article
- The fungistatic mechanism of benzaldehyde against the nematophagous fungus Arthrobotrys oligospora suggests a method for manipulating soil fungistasis.Communications biology · 2026Article
- Characterization and Migration Activity of Thermoresponsive Silk Fibroin-Aloe Vera Gel in Normal and Diabetic Fibroblasts.Gels (Basel, Switzerland) · 2026Article
- Astaxanthin enhances migration, Type I and Type III collagen production, and osteogenic differentiation in periodontal ligament fibroblasts under hyperglycemic conditions.BMC oral health · 2026Article
- Mechanistic and Therapeutic Insights into Nrf2-Mediated Redox Regulation in Periodontitis.Antioxidants (Basel, Switzerland) · 2026Review
- Phillyrin attenuates high glucose-induced ferroptosis in gingival cells through the activation of 5' adenosine monophosphate-activated protein kinase signaling.Journal of dental sciences · 2026Article
- Effect of glycaemic control on growth factor release andFrontiers in dental medicine · 2026Article
- Nicotinamide nucleotide transhydrogenase deficiency and genetic susceptibility to high glucose-mediated peritoneal injury in mice.Physiological reports · 2025Article
- Effects of High Glucose Concentrations on PC12 Cells: Possible Implications on Neurodegeneration.Current issues in molecular biology · 2025Article
- Time-Dependent Changes in Effects of Butyrate on Human Gingival Fibroblasts.Clinical and experimental dental research · 2025Article
- Mitochondrial aldehyde dehydrogenase restores the migratory capacity inhibited by high glucose-induced hyperosmolality.Scientific reports · 2025Article
- Dysfunctional crosstalk between macrophages and fibroblasts under LPS-infected and hyperglycemic environment in diabetic wounds.Scientific reports · 2025Article
35 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Delayed gingival wound healing is widely observed in periodontal patients with diabetes. However, the molecular mechanisms of the impaired function of gingival fibroblasts in diabetes remain unclear. The purpose of this study was to investigate changes in the properties of human gingival fibroblasts (HGFs) under high-glucose conditions. Primary HGFs were isolated from healthy gingiva and cultured with 5.5, 25, 50, and 75 mM glucose for 72 h. In vitro wound healing, 5-ethynyl-2'-deoxyuridine (EdU), and water-soluble tetrazolium salt (WST-8) assays were performed to examine cell migration and proliferation. Lactase dehydrogenase (LDH) levels were measured to determine cytotoxicity. The mRNA expression levels of oxidative stress markers were quantified by real-time PCR. Intracellular reactive oxygen species (ROS) were also measured in live cells. The antioxidant N-acetyl-l-cysteine (NAC, 1 mM) was added to evaluate the involvement of ROS in the glucose effect on HGFs. As a result, the in vitro wound healing assay showed that high glucose levels significantly reduced fibroblast migration and proliferation at 6, 12, 24, 36, and 48 h. The numbers of cells positive for EdU staining were decreased, as was cell viability, at 50 and 75 mM glucose. A significant increase in LDH was proportional to the glucose concentration. The mRNA levels of heme oxygenase-1 and superoxide dismutase-1 and ROS levels were significantly increased in HGFs after 72 h of exposure to 50 mM glucose concentration. The addition of NAC diminished the inhibitory effect of high glucose in the in vitro wound healing assay. The results of the present study show that high glucose impairs the proliferation and migration of HGFs. Fibroblast dysfunction may therefore be caused by high glucose-induced oxidative stress and may explain the delayed gingival wound healing in diabetic patients.
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