ArticlePloS one2018
Transcriptome sequencing and ITRAQ reveal the detoxification mechanism of Bacillus GJ1, a potential biocontrol agent for Huanglongbing.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 36 citations in OpenAlex.
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- Organic farming significantly improves microbial community structure, network complexity, and functional diversity in the Gannan navel orange orchard.BMC microbiology · 2025Article
- Exploring Aluminum Tolerance Mechanisms in Plants with Reference to Rice andPlants (Basel, Switzerland) · 2024Review
- Transcriptome analysis of Citrus sinensis reveals potential responsive events triggered by Candidatus Liberibacter asiaticus.Protoplasma · 2024Article
- Investigation of the anti-Huanglongbing effects using antimicrobial lipopeptide and phytohormone complex powder prepared fromFrontiers in microbiology · 2024Article
- Diversity Analysis and Function Prediction of Bacterial Communities in the Different Colored Pericarp ofInternational journal of molecular sciences · 2023Article
- Endophytes and Plant Extracts as Potential Antimicrobial Agents againstMicroorganisms · 2023Article
- Integrated proteomic and metabolomic analyses reveal significant changes in chloroplasts and mitochondria of pepper (Capsicum annuum L.) during Sclerotium rolfsii infection.Journal of microbiology (Seoul, Korea) · 2022Article
- An Overview of the Mechanisms Against "Frontiers in microbiology · 2022Review
- Bioprospecting Microbiome for Soil and Plant Health Management Amidst Huanglongbing Threat in Citrus: A Review.Frontiers in plant science · 2022Review
- Biocontrol Agents: Toolbox for the Screening of Weapons against MycotoxigenicJournal of fungi (Basel, Switzerland) · 2021Article
- Antibacterial Potential ofPlants (Basel, Switzerland) · 2021Article
- Comparative Transcriptomic Analysis of the Interaction betweenMicroorganisms · 2019Article
- Molecular Regulation of Host Defense Responses Mediated by Biological Anti-TMV Agent Ningnanmycin.Viruses · 2019Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Huanglongbing (HLB) is the most serious disease affecting citrus production worldwide. No HLB-resistant citrus varieties exist. The HLB pathogen Candidatus Liberibacter asiaticus is nonculturable, increasing the difficulty of preventing and curing the disease. We successfully screened the biocontrol agent Bacillus GJ1 for the control of HLB in nursery-grown citrus plants. RNA sequencing (RNA-seq) of the transcriptome and isobaric tags for relative and absolute quantification of the proteome revealed differences in the detoxification responses of Bacillus GJ1-treated and -untreated Ca. L. asiaticus-infected citrus. Phylogenetic tree alignment showed that GJ1 was classified as B. amyloliquefaciens. The effect of eliminating the HLB pathogen was measured using real-time quantitative polymerase chain reaction (qPCR) and PCR. The results indicate that the rate of detoxification reached 50% after seven irrigations, of plants with an OD600nm≈1 Bacillus GJ1 suspension. Most importantly, photosynthesis-antenna proteins, photosynthesis, plant-pathogen interactions, and protein processing in the endoplasmic reticulum were significantly upregulated (padj < 0.05), as shown by the KEGG enrichment analysis of the transcriptomes; nine of the upregulated genes were validated by qPCR. Transcription factor analysis of the transcriptomes was performed, and 10 TFs were validated by qPCR. Cyanoamino acid metabolism, regulation of autophagy, isoflavonoid biosynthesis, starch and sucrose metabolism, protein export, porphyrin and chlorophyll metabolism, and carotenoid biosynthesis were investigated by KEGG enrichment analysis of the proteome, and significant differences were found in the expression of the genes involved in those pathways. Correlation analysis of the proteome and transcriptome showed common entries for the significantly different expression of proteins and the significantly different expression of genes in the GO and KEGG pathways, respectively. The above results reveal important information about the detoxification pathways.
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