ArticleDrug testing and analysis2019
Oxytocin analysis from human serum, urine, and saliva by orbitrap liquid chromatography-mass spectrometry.
Article in Drug testing and analysis, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 43 citations in OpenAlex.
- Plasma pharmacokinetics of intravenous and intranasal oxytocin in nonpregnant adults.British journal of anaesthesia · 2025Trial
- Article
- Neurophysin-I dynamics upon different pituitary provocation tests in healthy participants.Endocrine connections · 2026Article
- The Oxytocin System and Implications for Oxytocin Deficiency in Hypothalamic-Pituitary Disease.Endocrine reviews · 2025Review
- Application of Lipophilic Prodrug Charge Masking Strategy to Obtain Novel, Potential Oxytocin Prodrugs.International journal of molecular sciences · 2025Article
- Development and validation of a highly-sensitive, quantitative LC-MS/MS assay to evaluate plasma oxytocin.Journal of mass spectrometry and advances in the clinical lab · 2025Article
- Oxytocin and women's health in midlife.The Journal of endocrinology · 2024Review
- A fit-for-purpose validation of a commercial radioimmunoassay for measurement of human peripheral oxytocin.Biochemistry and biophysics reports · 2024Article
- The Minhang Pediatric Biobank cohort study: protocol overview and baseline characteristics.BMC pediatrics · 2024Article
- Article
- Inaccuracies in plasma oxytocin extraction and enzyme immunoassay techniques.Comprehensive psychoneuroendocrinology · 2023Article
- Mother-Young Bonding: Neurobiological Aspects and Maternal Biochemical Signaling in Altricial Domesticated Mammals.Animals : an open access journal from MDPI · 2023Review
- Advances in human oxytocin measurement: challenges and proposed solutions.Molecular psychiatry · 2023Review
- The association between maternal body mass index and serial plasma oxytocin levels during labor.PloS one · 2023Observational
- Oxytocin and oxygen: the evolution of a solution to the 'stress of life'.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Review
- Oxytocin-a social peptide? Deconstructing the evidence.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Review
- ANGT_HUMAN[448-462], an Anorexigenic Peptide Identified Using Plasma Peptidomics.Journal of the Endocrine Society · 2022Article
- Oxytocin has 'tend-and-defend' functionality in group conflict across social vertebrates.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Review
- Approaches to Improve the Quantitation of Oxytocin in Human Serum by Mass Spectrometry.Frontiers in chemistry · 2022Article
- Salivary oxytocin in autistic patients and in patients with intellectual disability.Frontiers in psychiatry · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Oxytocin (OT) is a neurohormone that has gained interest recently due to its emerging role in cognition and social/emotional behaviors, including possibly depression and autism. OT is commonly measured using enzyme- or radio-based immunoassays (RIA, ELISA), which lack specificity or are complicated to perform and involve hazardous radioactive material. We have developed a high resolution accurate-mass (HRAM) liquid chromatography-mass spectrometry (LC-MS) method that separates interferences and selectively and accurately quantitates native OT from human serum, urine, and saliva after solid phase extraction. The doubly protonated OT ion m/z 562.25503 was selected for quantitation due its high signal intensity. With our method lower limit of detection (LLOD) of 5-25 pg/mL, we measured native OT in serum from pregnant women (16-24 pg/mL) and rats (350 pg/mL), and in serum, urine, and saliva from a healthy male after intranasal (IN) OT application of 100 IU and 20 IU and from a healthy post-menopausal female after IN OT application of 100 IU. Peak levels were detected in serum, urine, and saliva 15-30 minutes after each dose then decreased to below detection limits 1-2 hours thereafter. We were unable to detect native OT in serum from non-pregnant/non-lactating/non-medicated women due to levels known to occur below 5 pg/mL. The fast elimination of OT we found is in excellent agreement with the pharmacokinetics of OT in other studies. The effects on the central nervous system occurring after IN OT administration remains to be determined.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.