ArticleChemical science2015
Lab on a single microbead: an ultrasensitive detection strategy enabling microRNA analysis at the single-molecule level.
Article in Chemical science, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 76 citations in OpenAlex.
- MiR-199b-5p inhibition improves inflammation and oxidative stress damage in OA-induced non-alcoholic fatty liver disease through targeting SIRT1.BMC gastroenterology · 2025Article
- Recent advances in cascade isothermal amplification techniques for ultra-sensitive nucleic acid detection.Talanta · 2023Review
- A Novel Fluorescent Aptamer Sensor with DNAzyme Signal Amplification for the Detection of CEA in Blood.Sensors (Basel, Switzerland) · 2023Article
- Advances in optical counting and imaging of micro/nano single-entity reactors for biomolecular analysis.Analytical and bioanalytical chemistry · 2023Review
- Positive feedback drives a secondary nonlinear product burst during a biphasic DNA amplification reaction.The Analyst · 2022Article
- Single cell multi-miRNAs quantification with hydrogel microbeads for liver cancer cell subtypes discrimination.Chemical science · 2022Article
- Physical and chemical template-blocking strategies in the exponential amplification reaction of circulating microRNAs.Analytical and bioanalytical chemistry · 2020Article
- A mathematical model for a biphasic DNA amplification reaction.Journal of the Royal Society, Interface · 2019Article
- A new reporter design based on DNA origami nanostructures for quantification of short oligonucleotides using microbeads.Scientific reports · 2019Article
- Ultrasensitive detection of miRNA with an antimonene-based surface plasmon resonance sensor.Nature communications · 2019Article
- First characterization of a biphasic, switch-like DNA amplification.The Analyst · 2018Article
- Simultaneous visualization of the subfemtomolar expression of microRNA and microRNA target gene using HILO microscopy.Chemical science · 2017Article
- MnOACS applied materials & interfaces · 2017Article
- Comprehensive evaluation of molecular enhancers of the isothermal exponential amplification reaction.Scientific reports · 2016Article
- Asymmetric exponential amplification reaction on a toehold/biotin featured template: an ultrasensitive and specific strategy for isothermal microRNAs analysis.Nucleic acids research · 2016Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Detection of a single nucleic acid molecule is of great significance for both fundamental biochemistry studies and clinical diagnostics. By using microRNA (miRNA) as a model target, herein, we have developed a single-microbead-based sensing (SMBS) platform, which simply enables the detection of miRNA at the single-molecule level. In this strategy, an isothermal exponential amplification reaction (EXPAR) is rationally designed towards specific miRNAs and all products of the EXPAR are integrated onto a single microbead for signal amplification and fluorescence enrichment. This pushes the detection of miRNAs down to 1 aM in a 5 μL sample, corresponding to 3 copies of the miRNA molecule. This new strategy also affords high selectivity and it is capable of distinguishing among homologous miRNA family members even with a single-base difference. Due to its ultrahigh sensitivity and selectivity, the proposed SMBS platform has been successfully applied to the detection of miRNA extracted from a single cell.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.