Evidence map›Paper›PMID 30089864›Full record

ArticleScientific reports2018

Amplification and next generation sequencing of near full-length human enteroviruses for identification and characterisation from clinical samples.

Sonia R Isaacs, Ki Wook Kim, Junipearl X Cheng, Rowena A Bull, Sacha Stelzer-Braid, Fabio Luciani, William D Rawlinson, Maria E Craig

Abstract read
In one paragraph

Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed.

  1. Article
  2. Article
  3. Genetic Diversity of Human Enterovirus in Kazakhstan, during 2022.International journal of microbiology · 2024
    Article
  4. Article
  5. Review
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Review
  12. Article
  13. Review
  14. Article
  15. Next generation sequencing of human enterovirus strains from an outbreak of enterovirus A71 shows applicability to outbreak investigations.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2020
    Article
  16. Article
  17. Article
  18. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Sonia R IsaacsSchool of Women's and Children's Health, Faculty of Medicine, University of New South Wales, Sydney, NSW, 2052, Australia.
Ki Wook KimSchool of Women's and Children's Health, Faculty of Medicine, University of New South Wales, Sydney, NSW, 2052, Australia.
Junipearl X ChengSchool of Medical Sciences, Faculty of Medicine, University of New South Wales, Sydney, NSW, 2052, Australia.
Rowena A BullSchool of Medical Sciences, Faculty of Medicine, University of New South Wales, Sydney, NSW, 2052, Australia.
Sacha Stelzer-BraidVirology Research Laboratory, Prince of Wales Hospital, Sydney, NSW, 2031, Australia.
Fabio LucianiSchool of Medical Sciences, Faculty of Medicine, University of New South Wales, Sydney, NSW, 2052, Australia.
William D RawlinsonSchool of Women's and Children's Health, Faculty of Medicine, University of New South Wales, Sydney, NSW, 2052, Australia.
Maria E CraigSchool of Women's and Children's Health, Faculty of Medicine, University of New South Wales, Sydney, NSW, 2052, Australia. m.craig@unsw.edu.au.ORCID 0000-0001-6004-576X

Funding

Department of Health | National Health and Medical Research Council (NHMRC) APP1044694Department of Health | National Health and Medical Research Council (NHMRC) APP1045777Department of Health | National Health and Medical Research Council (NHMRC) APP510258Diabetes Australia Research Trust (DART) RG161637
6 · The paper itself

Abstract

More than 100 different enterovirus (EV) genotypes infect humans and contribute to substantial morbidity. However, current methods for characterisation of full-length genomes are based on Sanger sequencing of short genomic regions, which are labour-intensive and do not enable comprehensive characterisation of viral populations. Here, we describe a simple and sensitive protocol for the amplification and sequencing of near full-length genomes of human EV species using next generation sequencing. EV genomes were amplified from 89% of samples tested, with C

Indexed as

EnterovirusEnterovirus InfectionsGenome, ViralGenotypeHigh-Throughput Nucleotide SequencingHumansNucleic Acid Amplification TechniquesProspective Studies

Identifiers

PMID30089864
PMCPMC6082906

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.