ArticleBiology open2018
Biochemical and histological evidence of deteriorated bioprosthetic valve leaflets: the accumulation of fibrinogen and plasminogen.
Article in Biology open, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 49 citations in OpenAlex.
- Proteomic and degradomic signatures of extracellular matrix remodeling in calcific aortic valve stenosis.Molecular medicine (Cambridge, Mass.) · 2026Article
- Novel biodegradable device for transcatheter closure of atrial septal defects.Scientific reports · 2026Article
- Enzyme-responsive hydrogel coating forTheranostics · 2026Article
- Bioprosthetic heart valve degeneration: new insights into structural remodeling and calcification.Frontiers in cardiovascular medicine · 2026Review
- Ectopic Calcification in Congenital Heart Surgery: A Material-Centric Review.Pediatric cardiology · 2025Review
- The importance of tissue science and valve design in relation to durability and hemodynamics of the DurAVR aortic heart valve.Frontiers in cardiovascular medicine · 2025Review
- Structural valve deterioration is primarily caused by cyclic fatigue but not immune rejection.Frontiers in immunology · 2025Article
- Who Lives Longer, the Valve or the Patient? The Dilemma of TAVI Durability and How to Optimize Patient Outcomes.Journal of clinical medicine · 2024Review
- Anti-adhesive Properties of Epoxy-Treated Xenopericardium Modified with Polyvinyl Alcohol:Sovremennye tekhnologii v meditsine · 2024Article
- Low-Grade Inflammation: A Familiar Factor in Cardiovascular Diseases.JACC. Basic to translational science · 2023Article
- Embedding and Backscattered Scanning Electron Microscopy (EM-BSEM) Is Preferential over Immunophenotyping in Relation to Bioprosthetic Heart Valves.International journal of molecular sciences · 2023Article
- Graft rejection in paediatric congenital heart disease.Translational pediatrics · 2023Review
- Soft Biomimetic Approach for the Development of Calcinosis-Resistant Glutaraldehyde-Fixed Biomaterials for Cardiovascular Surgery.Biomimetics (Basel, Switzerland) · 2023Article
- Bioprosthetic Valve Deterioration: Accumulation of Circulating Proteins and Macrophages in the Valve Interstitium.JACC. Basic to translational science · 2023Article
- Fibrin deposition on bovine pericardium tissue used for bioprosthetic heart valve drives its calcification.Frontiers in cardiovascular medicine · 2023Article
- Hybrid heart valves with VEGF-loaded zwitterionic hydrogel coating for improved anti-calcification and re-endothelialization.Materials today. Bio · 2022Article
- Article
- Patient-specific fluid simulation of transcatheter mitral valve replacement in mitral annulus calcification.Frontiers in cardiovascular medicine · 2022Article
- Mechanisms and Drug Therapies of Bioprosthetic Heart Valve Calcification.Frontiers in pharmacology · 2022Review
- Detecting native and bioprosthetic aortic valve disease usingJournal of nuclear cardiology : official publication of the American Society of Nuclear Cardiology · 2021Review
Corrections and comments
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Authors and funding
16 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Calcification of bioprosthetic valves (BVs) implanted in aortic position can result in gradual deterioration and necessitate aortic valve replacement. The molecular mechanism of calcium deposition on BV leaflets has been investigated, but remains to be fully elucidated. The present study aimed to identify explanted bioprosthetic valve (eBV)-specific proteins using a proteomics approach and to unveil their biochemical and histological involvements in calcium deposition on BV leaflets. Calcification, fibrosis, and glycosylation of the valves were histologically assessed using Von Kossa, Masson's Trichrome and Alcian Blue staining, as well as immunostaining. Protein expression in the explanted biological valves was analysed using proteomics and western blotting. In a histological evaluation, αSMA-positive myofibroblasts were not observed in eBV, whereas severe fibrosis occurred around calcified areas. SDS-PAGE revealed three major bands with considerably increased intensity in BV leaflets that were identified as plasminogen and fibrinogen gamma chain (100 kDa), and fibrinogen beta chain (50 and 37 kDa) by mass analysis. Immunohistochemistry showed that fibrinogen β-chain was distributed throughout the valve tissue. On the contrary, plasminogen was strongly stained in CD68-positive macrophages, as evidenced by immunofluorescence. The results suggest that two important blood coagulation-related proteins, plasminogen and fibrinogen, might affect the progression of BV degeneration.
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Registered trials
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