Evidence map›Paper›PMID 30089262›Full record

ArticleCell reports2018

The Spectrin-Actin-Based Periodic Cytoskeleton as a Conserved Nanoscale Scaffold and Ruler of the Neural Stem Cell Lineage.

Meghan Hauser, Rui Yan, Wan Li, Nicole A Repina, David V Schaffer, Ke Xu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.

0numbers the graph read from it
0cells of the map it votes in
29citing papers in PubMed
6.2field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

29 citing papers in PubMed, 48 citations in OpenAlex.

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  17. Load adaptation by endocytic actin networks.Molecular biology of the cell · 2022
    Article
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  19. The role of the membrane-associated periodic skeleton in axons.Cellular and molecular life sciences : CMLS · 2021
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Meghan HauserDepartment of Chemistry, University of California, Berkeley, Berkeley, CA 94720, USA.
Rui YanDepartment of Chemistry, University of California, Berkeley, Berkeley, CA 94720, USA.
Wan LiDepartment of Chemistry, University of California, Berkeley, Berkeley, CA 94720, USA.
Nicole A RepinaDepartment of Bioengineering, University of California, Berkeley, Berkeley, CA 94720, USA.
David V SchafferDepartment of Bioengineering, University of California, Berkeley, Berkeley, CA 94720, USA; Department of Chemical and Biomolecular Engineering, University of California, Berkeley, Berkeley, CA 94720, USA; Helen Wills Neuroscience Institute, University of California, Berkeley, Berkeley, CA 94720, USA; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA.
Ke XuDepartment of Chemistry, University of California, Berkeley, Berkeley, CA 94720, USA; Helen Wills Neuroscience Institute, University of California, Berkeley, Berkeley, CA 94720, USA; Division of Molecular Biophysics and Integrated Bioimaging, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA; Chan Zuckerberg Biohub, San Francisco, CA 94158, USA. Electronic address: xuk@berkeley.edu.
University of California, Berkeley · USLawrence Berkeley National Laboratory · US

Funding

Optogenetic Characterization and Control of Stem Cell SignalingR01NS087253 · NINDS · UNIVERSITY OF CALIFORNIA BERKELEY · PI KANE, RAVI S., SCHAFFER, DAVID V · 2014 to 2018
$1.7M
NINDS NIH HHS R01 NS087253
6 · The paper itself

Abstract

Through three-dimensional STORM super-resolution microscopy, we resolve the spectrin-actin-based membrane cytoskeleton of neural stem cells (NSCs) and NSC-derived neurons, astrocytes, and oligodendrocytes. We show that undifferentiated NSCs are capable of forming patches of locally periodic, one-dimensional (1D) membrane cytoskeleton with ∼180 nm periodicity. Such periodic structures become increasingly ordered and long-ranging as the NSCs mature into terminally differentiated neuronal and glial cell types, and, during this process, distinct 1D periodic "strips" dominate the flat 2D membranes. Moreover, we report remarkable alignment of the periodic cytoskeletons between abutting cells at axon-axon and axon-oligodendrocyte contacts and identify two adhesion molecules, neurofascin and L1CAM, as candidates to drive this nanoscale alignment. We thus show that a conserved 1D periodic membrane cytoskeletal motif serves as a nanoscale scaffold and ruler to mediate the physical interactions between cell types of the NSC lineage.

Indexed as

Actin CytoskeletonCell LineageHumansNeural Stem CellsSpectrinSpectrincell adhesion moleculecell-cell interactionmembrane cytoskeletonneural stem cellperiodic cytoskeletonspectrin-actin cytoskeletonsuper-resolution microscopy

Identifiers

PMID30089262
PMCPMC6119594
OpenAlexW2887350925

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.