ArticleCytometry. Part A : the journal of the International Society for Analytical Cytology2018
ChromaWizard: An open source image analysis software for multicolor fluorescence in situ hybridization analysis.
Article in Cytometry. Part A : the journal of the International Society for Analytical Cytology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 9 citations in OpenAlex.
- MetaChrome: an open-source, user-friendly tool for automated metaphase chromosome analysis.Methods (San Diego, Calif.) · 2026Article
- MetaChrome: An Open-Source, User-Friendly Tool for Automated Metaphase Chromosome Analysis.bioRxiv : the preprint server for biology · 2025Article
- A reliable technique for karyotyping mouse oocytes prepared by a gradual fixation/air-drying method followed by multicolour FISH.Biology open · 2023Article
- Birth of mice from meiotically arrested spermatocytes following biparental meiosis in halved oocytes.EMBO reports · 2022Article
- Article
- Imaging approaches for chromosome structures.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2021Review
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Multicolor image analysis finds its applications in a broad range of biological studies. Specifically, multiplex fluorescence in situ hybridization (M-FISH) for chromosome painting facilitates the analysis of individual chromosomes in complex metaphase spreads and is widely used to detect both numerical and structural aberrations. While this is well established for human and mouse karyotypes, for which species sophisticated software and analysis tools are available, other organisms and species are less well served. Commercially available software is proprietary and not easily adaptable to other karyotypes. Therefore, a publically available open source software that combines flexibility and customizable functionalities is needed. Here we present such a tool called "ChromaWizard" which is based on popular scientific image analysis libraries (OpenCV, scikit-image, and NumPy). We demonstrate its functionality on the example of primary Chinese hamster (Cricetulus griseus) fibroblasts metaphase spreads and on Chinese Hamster Ovary cell lines known for the large number of chromosomal rearrangements. The application can be easily adapted to any kind of available labeling kits and is independent of the used organism and instrumentation. It allows direct inspection of the original hybridization signals and enables either manual or automatic assignment of colors, making it a functional and versatile tool that can be used also for other multicolor applications.
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