ArticleGenetics and molecular biology
pGVG: a new Gateway-compatible vector for transformation of sugarcane and other monocot crops.
Article in Genetics and molecular biology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Enhanced expression of theGM crops & food · 2026Article
- Engineering glyphosate tolerance in sugarcane through overexpression of a mutated version of its nativeGM crops & food · 2026Article
- Transgenic sugarcane harboringFrontiers in plant science · 2026Article
- Silencing ScGUX2 reduces xylan glucuronidation and improves biomass saccharification in sugarcane.Plant biotechnology journal · 2024Article
- CRISPR technology towards genome editing of the perennial and semi-perennial crops citrus, coffee and sugarcane.Frontiers in plant science · 2023Review
- Reduction of ethylene biosynthesis in sugarcane induces growth and investment in the non-enzymatic antioxidant apparatus.Plant cell reports · 2022Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The successful development of genetically engineered monocots using Agrobacterium-mediated transformation has created an increasing demand for compatible vectors. We have developed a new expression vector, pGVG, for efficient transformation and expression of different constructs for gene overexpression and silencing in sugarcane. The pCAMBIA2300 binary vector was modified by adding Gateway recombination sites for fast gene transfer between vectors and the maize polyubiquitin promoter Ubi-1 (ZmUbi1), which is known to drive high gene expression levels in monocots. Transformation efficiency using the pGVG vector reached up to 14 transgenic events per gram of transformed callus. Transgenic plants expressing the β-glucuronidase (GUS) reporter gene from pGVG showed high levels of GUS activity. qRT-PCR evaluations demonstrated success for both overexpression and hairpin-based silencing cassettes. Therefore, pGVG is suitable for plant transformation and subsequent applications for high-throughput production of stable transgenic sugarcane. The use of an expression cassette based on the ZmUbi1 promoter opens the possibility of using pGVG in other monocot species.
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Registered trials
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