Evidence map›Paper›PMID 30088235›Full record

ArticleNeurochemical research2018

TLR2 Ligand Pam3CSK4 Regulates MMP-2/9 Expression by MAPK/NF-κB Signaling Pathways in Primary Brain Microvascular Endothelial Cells.

Hongyan Zhu, Rongrong Dai, Youquan Zhou, Hao Fu, Qiang Meng

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Neurochemical research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed, 2 pooled it
1.7field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed, 2 syntheses or guidelines pooled it, 34 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 1 country.

Hongyan ZhuFaculty of Environmental Science and Engineering, Kunming University of Science and Technology, Kunming, 650093, Yunnan Province, China.
Rongrong DaiDepartment of Clinical Laboratory, The First Affiliated Hospital of Kunming Medical University, Kunming, 650034, Yunnan Province, China.
Youquan ZhouDepartment of Clinical Laboratory, The Third Affiliated Hospital of Kunming Medical University, Kunming, 650118, Yunnan Province, China.
Hao FuDepartment of Neurology, The First People's Hospital of Yunnan Province, Kunming, 650031, Yunnan Province, China.
Qiang MengDepartment of Neurology, The First People's Hospital of Yunnan Province, Kunming, 650031, Yunnan Province, China. mq301@sina.com.
First People's Hospital of Yunnan Province · CNKunming Medical University · CNKunming University of Science and Technology · CN

Funding

National Natural Science Foundation of China 81460188the Applied Basic Combined Special Project of the Department of Science and Technology of Yunnan Province and Kunming Medical University 2013FB201the Applied Basic Research Project in Yunnan Province of China 2011FB148the Health System Leaders of Yunnan Province of China D-201235the Young and Middle-Aged Academic and Technological Leaders and Reserved Talents of Yunnan Province of China 2012HB028
6 · The paper itself

Abstract

Blood-brain barrier (BBB) destruction is associated with a variety of neurological diseases. Brain microvascular endothelial cells (BMECs) are the key constituent of BBB. Both matrix metalloproteinases-2/9 (MMP-2/9) and toll-like receptor-2 (TLR2) are coexpressed in BMECs and have been shown to play important roles in BBB breakdown. It is unknown whether TLR2 can regulate MMP-2/9 in BMECs. In this study, Pam3CSK4 was used to activate TLR2, and the expression of MMP-2/9 and tight junctions (TJs) in BBB was measured by quantitative real-time PCR and western blotting. Phosphoproteins were determined by western blotting. The inhibitors of mitogen-activated protein kinases (MAPKs) and NF-κB were used to identify the signaling pathways by which TLR2 regulates the expression of MMP-2/9 in BMECs. This study showed that Pam3CSK4 upregulated the mRNA and protein expression of MMP-9 and downregulated MMP-2 and TJ expression in BMECs simultaneously. Pam3CSK4 also induced the phosphorylation of MAPKs and NF-κB signaling pathways in BMECs. MMP-9 expression was found to decrease by pretreatment with inhibitors of ERK1/2 and JNK but not p38. However, the mRNA and protein expression of MMP-2 and MMP-9 increased after addition of a NF-κB inhibitor. Our results indicated that Pam3CSK4 was able to upregulate MMP-9 expression through ERK1/2 and JNK signaling pathways, but the NF-κB signaling pathway negatively regulated the effect of TLR2 on MMP-2 and MMP-9 expression in BMECs. The finding provides novel insight into the molecular mechanism of MMP-2/9 expression in BMECs.

Indexed as

AnimalsBrainEndothelial CellsLipopeptidesMatrix Metalloproteinase 2Matrix Metalloproteinase 9Mitogen-Activated Protein KinasesNF-kappa BPhosphorylationRats, Sprague-DawleySignal TransductionToll-Like Receptor 2LipopeptidesMatrix Metalloproteinase 2Matrix Metalloproteinase 9Mitogen-Activated Protein KinasesMmp2 protein, ratMmp9 protein, ratNF-kappa BPam(3)CSK(4) peptideTlr2 protein, ratToll-Like Receptor 2Brain microvascular endothelial cellsMatrix metalloproteinaseMitogen-activated protein kinasesPam3CSK4Toll-like receptor-2

Identifiers

PMID30088235
PMCPMC6182369
OpenAlexW2887314695

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.