Evidence map›Paper›PMID 30088201›Full record

ArticleMolecular biology reports2018

Involvement of a coumarin analog AD-013 in the DNA damage response pathways in MCF-7 cells.

Angelika Długosz, Joanna Drogosz, Dariusz Deredas, Tomasz Janecki, Anna Janecka

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular biology reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 43% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Angelika DługoszDepartment of Biomolecular Chemistry, Faculty of Medicine, Medical University of Lodz, Mazowiecka 6/8, 92-215, Lodz, Poland.
Joanna DrogoszDepartment of Biomolecular Chemistry, Faculty of Medicine, Medical University of Lodz, Mazowiecka 6/8, 92-215, Lodz, Poland.
Dariusz DeredasInstitute of Organic Chemistry, Faculty of Chemistry, Lodz University of Technology, Lodz, Poland.
Tomasz JaneckiInstitute of Organic Chemistry, Faculty of Chemistry, Lodz University of Technology, Lodz, Poland.
Anna JaneckaDepartment of Biomolecular Chemistry, Faculty of Medicine, Medical University of Lodz, Mazowiecka 6/8, 92-215, Lodz, Poland. anna.janecka@umed.lodz.pl.ORCID http://orcid.org/0000-0001-5178-4464
Medical University of Lodz · PLLodz University of Technology · PL

Funding

Narodowe Centrum Nauki DEC-2012/07/B/ST5/02006Uniwersytet Medyczny w Lodzi 502-14-300Uniwersytet Medyczny w Lodzi 503/1-156-02/503-11-02
6 · The paper itself

Abstract

Coumarin is a plant-derived compound but as such has no medical uses. Several synthetic coumarin analogs have been shown to possess anti-proliferative activity and to induce apoptosis in cancer cells. Here, we explored DNA damage responses in MCF-7 cells treated with our novel synthetic hybrid compound AD-013, which integrates a coumarin moiety and an α-methylene-δ-lactone motif. The mRNA expression of several genes engaged in DNA-damage-induced responses was assessed by quantitative real-time PCR. The protein levels of a few members of phosphoinositide-3-kinases family (ATM, ATR and DNA-PK) and BRCA1 were assessed by ELISA, while p53 was evaluated by western blot method. AD-013 down-regulated DNA-PK gene expression but increased the level of ATM/ATR and p53. The new analog completely inhibited BRCA1 and greatly decreased the activity of BRCA1 protein, engaged in DNA damage repair. Exposure of MCF-7 cells to a coumarin analog AD-013 led to DNA damage and decreased expression of several repair-associated genes.

Indexed as

DNA DamageAntineoplastic AgentsAtaxia Telangiectasia Mutated ProteinsBRCA1 ProteinBreast NeoplasmsCell ProliferationCell SurvivalCoumarinsDNA-Activated Protein KinaseFemaleGene Expression Regulation, NeoplasticHumansMCF-7 CellsNuclear ProteinsTumor Suppressor Protein p53AD-013Antineoplastic AgentsAtaxia Telangiectasia Mutated ProteinsATM protein, humanATR protein, humanBRCA1 ProteinBRCA1 protein, humanCoumarinsDNA-Activated Protein KinaseNuclear ProteinsPRKDC protein, humanTP53 protein, humanTumor Suppressor Protein p53ApoptosisATMBRCA1DNA damageP53

Identifiers

PMID30088201
PMCPMC6156763
OpenAlexW2887643324

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.