Evidence map›Paper›PMID 30087387›Full record

ArticleScientific reports2018

Next-generation amplicon sequencing identifies genetically diverse human astroviruses, including recombinant strains, in environmental waters.

Akihiko Hata, Masaaki Kitajima, Eiji Haramoto, Suntae Lee, Masaru Ihara, Charles P Gerba, Hiroaki Tanaka

Open access · goldAbstract read
In one paragraph

Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed
2.3field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed, 42 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Virus evolution · 2025
    Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. Review
  14. Review
  15. Article
  16. Review
  17. Review
  18. Article
  19. Review
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 2 countries.

Akihiko HataResearch Center for Environmental Quality Management, Kyoto University, Shiga, Japan. hata@env.t.u-tokyo.ac.jp.ORCID 0000-0002-9372-5013
Masaaki KitajimaDivision of Environmental Engineering, Faculty of Engineering, Hokkaido University, Hokkaido, Japan.
Eiji HaramotoInterdisciplinary Center for River Basin Environment, Graduate Faculty of Interdisciplinary Research, University of Yamanashi, Yamanashi, Japan.ORCID 0000-0002-0126-0651
Suntae LeeResearch Center for Environmental Quality Management, Kyoto University, Shiga, Japan.ORCID 0000-0003-0013-8002
Masaru IharaResearch Center for Environmental Quality Management, Kyoto University, Shiga, Japan.
Charles P GerbaDepartment of Soil, Water and Environmental Science, The University of Arizona, Tucson, Arizona, USA.
Hiroaki TanakaResearch Center for Environmental Quality Management, Kyoto University, Shiga, Japan.
Kyoto University · JPHokkaido University · JPUniversity of Arizona · USUniversity of Yamanashi · JP

Funding

Japan Society for the Promotion of Science (JSPS) 17H03332Japan Society for the Promotion of Science (JSPS) JP 26289182Japan Society for the Promotion of Science (JSPS) JP 26820222
6 · The paper itself

Abstract

Human astroviruses are associated with gastroenteritis and known to contaminate water environments. Three different genetic clades of astroviruses are known to infect humans and each clade consists of diverse strains. This study aimed to determine the occurrence and genetic diversity of astrovirus strains in water samples in different geographical locations, i.e., influent and effluent wastewater samples (n = 24 each) in Arizona, U.S., and groundwater (n = 37) and river water (n = 14) samples collected in the Kathmandu Valley, Nepal, using next-generation amplicon sequencing. Astrovirus strains including rare types (types 6 and 7 classical human astroviruses), emerging type (type 5 VA-astroviruses), and putative recombinants were identified. Feline astrovirus strains were collaterally identified and recombination between human and feline astroviruses was suggested. Classical- and VA-astroviruses seemed to be prevalent during cooler months, while MLB-astroviruses were identified only during warmer months. This study demonstrated the effectiveness of next-generation amplicon sequencing for identification and characterization of genetically diverse astrovirus strains in environmental water.

Indexed as

Genetic VariationRecombination, GeneticAnimalsArizonaCatsEnvironmental MicrobiologyGastroenteritisGenotypeHigh-Throughput Nucleotide SequencingHumansMamastrovirusNepalPhylogenySeasonsWastewaterWastewater

Identifiers

PMID30087387
PMCPMC6081416
OpenAlexW2885092108

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.