Evidence map›Paper›PMID 30087266›Full record

ArticleSensors (Basel, Switzerland)2018

Q3: A Compact Device for Quick, High Precision qPCR.

Marco Cereda, Alessandro Cocci, Davide Cucchi, Lillo Raia, Danilo Pirola, Lorenzo Bruno, Pietro Ferrari, Valentina Pavanati, Giorgia Calisti, Francesco Ferrara and 2 more

Open access · goldAbstract read
In one paragraph

Article in Sensors (Basel, Switzerland), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
1.0field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 25 citations in OpenAlex.

  1. Article
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  4. Q3 lab-on-chip real-time PCR for the diagnosis ofJournal of clinical microbiology · 2024
    Article
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  6. Article
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  8. Article
  9. Miniaturized Real-Time PCR systems for SARS-CoV-2 detection at the Point-of-Care.Clinica chimica acta; international journal of clinical chemistry · 2022
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 4 institutions in 3 countries.

Marco CeredaSTMicroelectronics S.r.l., I-20864 Agrate Brianza, Monza Brianza, Italy. marco.cereda@st.com.ORCID 0000-0002-5868-8616
Alessandro CocciSTMicroelectronics S.r.l., I-20864 Agrate Brianza, Monza Brianza, Italy. alessandro.cocci@st.com.
Davide CucchiSTMicroelectronics S.r.l., I-20864 Agrate Brianza, Monza Brianza, Italy. davide.cucchi@st.com.
Lillo RaiaSTMicroelectronics S.r.l., I-20864 Agrate Brianza, Monza Brianza, Italy. lillo.raia@st.com.
Danilo PirolaSTMicroelectronics S.r.l., I-20864 Agrate Brianza, Monza Brianza, Italy. danilo.pirola@st.com.
Lorenzo BrunoSTMicroelectronics S.r.l., I-20864 Agrate Brianza, Monza Brianza, Italy. lorenzo.bruno@st.com.
Pietro FerrariSTMicroelectronics S.r.l., I-20864 Agrate Brianza, Monza Brianza, Italy. pietro.ferrari@st.com.
Valentina PavanatiSTMicroelectronics S.r.l., I-20864 Agrate Brianza, Monza Brianza, Italy. valentina.pavanati@st.com.
Giorgia CalistiCNR IMM-MDM, I-20864 Agrate Brianza, Monza Brianza, Italy. giorgia.calisti-ext@st.com.
Francesco FerraraSTMicroelectronics S.r.l., I-73100 Lecce, Italy. francesco.ferrara@st.com.ORCID 0000-0002-7290-9542
Alessandro P BramantiSTMicroelectronics S.r.l., I-73100 Lecce, Italy. alessandro.bramanti@st.com.ORCID 0000-0001-9266-9075
Marco A BianchessiSTMicroelectronics S.r.l., I-20864 Agrate Brianza, Monza Brianza, Italy. marco.bianchessi@st.com.
STMicroelectronics (Czechia) · CZSTMicroelectronics (Italy) · ITSTMicroelectronics (Switzerland) · CHTecnologie Avanzate (Italy) · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

An accurate and easy-to-use Q3 system for on-chip quantitative real-time Polymerase Chain Reaction (qPCR) is hereby demonstrated, and described in detail. The qPCR reactions take place inside a single-use Lab-on-a-Chip with multiple wells, each with 5 to 15 µL capacity. The same chip hosts a printed metal heater coupled with a calibrated sensor, for rapid and accurate temperature control inside the reaction mixture. The rest of the system is non-disposable and encased in a 7 × 14 × 8.5 (height) cm plastic shell weighing 300 g. Included in the non-disposable part is a fluorescence read-out system featuring up to four channels and a self-contained control and data storage system, interfacing with an external user-friendly software suite. Hereby, we illustrate the engineering details of the Q3 system and benchmark it with seamlessly ported testing protocols, showing that Q3 equals the performance of standard commercial systems. Overall, to the best of our knowledge, this is one of the most mature general-purpose systems for on-chip qPCR currently available.

Indexed as

Lab-On-A-Chip DevicesReal-Time Polymerase Chain ReactionTemperatureLab-on-a-Chiplow-cost devicesminiaturizationon-chip diagnosispoint-of-care testsQ3qPCRreal-time PCR

Identifiers

PMID30087266
PMCPMC6111627
OpenAlexW2887327574

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.