ArticleParasites & vectors2018
Molecular survey of piroplasm species from selected areas of China and Pakistan.
Article in Parasites & vectors, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 35 citations in OpenAlex.
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- Exploring the Coinfection and Genetic Diversity of Multiple Tick-Borne Pathogens in Livestock Population of Punjab, Pakistan.Transboundary and emerging diseases · 2024Article
- Molecular epidemiology, associated risk factors, and phylogeny ofIranian journal of veterinary research · 2023Article
- Molecular Characterization and Assessment of Risk Factors Associated withMicroorganisms · 2022Article
- Molecular Detection and Characterization ofMicroorganisms · 2022Article
- Molecular Epidemiology ofAnimals : an open access journal from MDPI · 2021Article
- Molecular detection of Theileria species, Anaplasma species, Candidatus Mycoplasma haemobos, Trypanosoma evansi and first evidence of Theileria sinensis-associated bovine anaemia in crossbred Kedah-Kelantan x Brahman cattle.BMC veterinary research · 2021Article
- Molecular epidemiology of Theileria annulata infection of cattle in Layyah District, Pakistan.Experimental & applied acarology · 2021Article
- Molecular Detection of Theileria annulata among Dairy Cattle and Vector Ticks in the Herat Area, Afghanistan.Archives of Razi Institute · 2021Article
- Knowledge, attitude and perception of bovine piroplasmosis by cattle owners in Constantine, North-East of Algeria, using participatory epidemiology.Tropical animal health and production · 2021Article
- Epidemiology and molecular characterization of Theileria annulata in cattle from central Khyber Pakhtunkhwa, Pakistan.PloS one · 2021Article
- Targeted Next-Generation Sequencing and Informatics as an Effective Tool to Establish the Composition of Bovine Piroplasm Populations in Endemic Regions.Microorganisms · 2020Article
- Genomic Profiling for Piroplasms in Feeding Ixodid Ticks in the Eastern Cape, South Africa.Pathogens (Basel, Switzerland) · 2020Article
- Clinical Pathology, Immunopathology and Advanced Vaccine Technology in Bovine Theileriosis: A Review.Pathogens (Basel, Switzerland) · 2020Review
- Bovine ticks harbour a diverse array of microorganisms in Pakistan.Parasites & vectors · 2020Article
- First molecular survey of piroplasm species in cattle from Kyrgyzstan.Parasitology research · 2019Article
- Establishment and Expression of Cytokines in aGenes · 2019Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundPiroplasmosis is an important animal disease that is a major constraint to the development of the livestock industry, often resulting in significant economic losses. Therefore, there is an urgent need to further understand the etiology of this and other tick-borne infections.
methodsBlood samples were collected from tick-infested animals from the Chakwal, Jhang, and Faisalabad districts of Punjab, Pakistan and from peri-urban areas around Hohhot, Inner Mongolia, China to investigate the presence of Babesia and Theileria species. In total, 450 blood samples were collected with FTA cards from cattle of the study areas of Pakistan; the genomic (g)DNA of one hundred and twenty samples from cattle in Inner Mongolia were provided by the Lanzhou Veterinary Research Institute, China. Following the extraction of gDNA, the 18S rRNA gene (V4 hypervariable region) of piroplasms was amplified in all samples using semi-nested PCR. Positively identified samples were sequenced for the identification of Theileria and Babesia species. The partial full-length sequence of 18S rDNA was amplified for species confirmation of Theileria-positive samples, whereas the RAP-1c gene was amplified for Babesia bigemina-positive samples.
resultsSemi-nested PCR results revealed that 144 (25.26%) samples were positive for piroplasms. Theileria annulata was the most prevalent species (115/144; 20.17%), followed by Theileria orientalis (16/144; 2.80%). Among Babesia, the only species recorded was Babesia bigemina (13/144; 2.28%).
conclusionThe present study reveals new data on the prevalence of piroplasm species in bovine populations of selected areas of China and Pakistan and their phylogenetic relationships. It is also the first detailed report of T. orientalis from native animals in Pakistan.
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