Evidence map›Paper›PMID 30086719›Full record

ArticleBMC genomics2018

Proteomic and evolutionary analyses of sperm activation identify uncharacterized genes in Caenorhabditis nematodes.

Katja R Kasimatis, Megan J Moerdyk-Schauwecker, Nadine Timmermeyer, Patrick C Phillips

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC genomics, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 47% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Katja R KasimatisInstitute of Ecology and Evolution, University of Oregon, 5289 University of Oregon, Eugene, OR, 97403-5289, USA.
Megan J Moerdyk-SchauweckerInstitute of Ecology and Evolution, University of Oregon, 5289 University of Oregon, Eugene, OR, 97403-5289, USA.
Nadine TimmermeyerInstitute of Ecology and Evolution, University of Oregon, 5289 University of Oregon, Eugene, OR, 97403-5289, USA.
Patrick C PhillipsInstitute of Ecology and Evolution, University of Oregon, 5289 University of Oregon, Eugene, OR, 97403-5289, USA. pphil@uoregon.edu.
University of Oregon · US

Funding

Graduate Training in Genetics Administrative Supplement - Career Curriculum DevelopmentT32GM007413 · NIGMS · UNIVERSITY OF OREGON · PI GUILLEMIN, KAREN J · 1985 to 2022
$7.8M
Deterministic and stochastic effects of diet on demography Administrative Supplement to AG049396R01AG049396 · NIA · UNIVERSITY OF OREGON · PI PHILLIPS, PATRICK C. · 2015 to 2019
$1.8M
Systems genetics of natural variation in stress response pathwaysR01GM102511 · NIGMS · UNIVERSITY OF OREGON · PI PHILLIPS, PATRICK C. · 2015 to 2018
$1.1M
National Institutes of Health R01AG049396National Institutes of Health R01GM102511National Institutes of Health T32GM007413NIA NIH HHS R01 AG049396NIGMS NIH HHS R01 GM102511NIGMS NIH HHS T32 GM007413
6 · The paper itself

Abstract

backgroundNematode sperm have unique and highly diverged morphology and molecular biology. In particular, nematode sperm contain subcellular vesicles known as membranous organelles that are necessary for male fertility, yet play a still unknown role in overall sperm function. Here we take a novel proteomic approach to characterize the functional protein complement of membranous organelles in two Caenorhabditis species: C. elegans and C. remanei.

resultsWe identify distinct protein compositions between membranous organelles and the activated sperm body. Two particularly interesting and undescribed gene families-the Nematode-Specific Peptide family, group D and the here designated Nematode-Specific Peptide family, group F-localize to the membranous organelle. Both multigene families are nematode-specific and exhibit patterns of conserved evolution specific to the Caenorhabditis clade. These data suggest gene family dynamics may be a more prevalent mode of evolution than sequence divergence within sperm. Using a CRISPR-based knock-out of the NSPF gene family, we find no evidence of a male fertility effect of these genes, despite their high protein abundance within the membranous organelles.

conclusionsOur study identifies key components of this unique subcellular sperm component and establishes a path toward revealing their underlying role in reproduction.

Indexed as

AnimalsCaenorhabditisCaenorhabditis elegansEvolution, MolecularHelminth ProteinsMaleMultigene FamilyProteomicsSpecies SpecificitySpermatozoaHelminth ProteinsMolecular evolutionNematodesProteomicsSpermiogenesis

Identifiers

PMID30086719
PMCPMC6081950
OpenAlexW2951991821

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.