ArticleBMC cell biology2018
Novel roles for scleraxis in regulating adult tenocyte function.
Article in BMC cell biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Unravelling the Distinct Phenotype and Mechanosensitive Properties of Different Tendon Cell Populations.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Single-Step Extrusion Printing of Microgrooved Annulus Fibrosus Scaffolds via Patterned Nozzles.Journal of functional biomaterials · 2026Article
- Transcriptional Adaptations to Muscle Loading in a Murine Model of Achilles Tendinopathy.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2026Article
- Tendon mechanobiology in the context of tendon biofabrication.Frontiers in bioengineering and biotechnology · 2025Review
- Molecular mechanisms of mechanosensing and plasticity of tendons and ligaments.Journal of biochemistry · 2024Review
- Establishment of a Mouse Degenerative Model of Patellar Tendinopathy with Upregulation of Inflammation.International journal of molecular sciences · 2024Article
- Microfluidically Aligned Collagen to Maintain the Phenotype of Tenocytes In Vitro.Advanced healthcare materials · 2024Article
- The tendon unit: biochemical, biomechanical, hormonal influences.Journal of orthopaedic surgery and research · 2023Review
- Tendon-Specific Activation of Tenogenic Transcription Factors Enables Keeping Tenocytes' Identity In Vitro.International journal of molecular sciences · 2022Article
- TGF-β2 enhances expression of equine bone marrow-derived mesenchymal stem cell paracrine factors with known associations to tendon healing.Stem cell research & therapy · 2022Article
- Non-Viral Delivery of Gene Therapy to the Tendon.Polymers · 2022Review
- Optimizing Tenogenic Differentiation of Equine Adipose-Derived Mesenchymal Stem Cells (eq-ASC) Using TGFB3 Along with BMP Antagonists.Cell journal · 2022Article
- Tamoxifen Prevents Peritendinous Adhesions: A Preliminary Report.BioMed research international · 2022Article
- Analysis of the lncRNA-Associated Competing Endogenous RNA (ceRNA) Network for Tendinopathy.Genetics research · 2022Article
- 3D Biomimetic Scaffold for Growth Factor Controlled Delivery: An In-Vitro Study of Tenogenic Events on Wharton's Jelly Mesenchymal Stem Cells.Pharmaceutics · 2021Article
- Serum deprivation limits loss and promotes recovery of tenogenic phenotype in tendon cell culture systems.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2021Article
- Impact of isolation method on cellular activation and presence of specific tendon cell subpopulations during in vitro culture.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Article
- Article
- Metabolic Regulation of Tendon Inflammation and Healing Following Injury.Current rheumatology reports · 2021Review
- Influence of microcurrent on the modulation of remodelling genes in a wound healing assay.Molecular biology reports · 2021Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTendinopathies are common and difficult to resolve due to the formation of scar tissue that reduces the mechanical integrity of the tissue, leading to frequent reinjury. Tenocytes respond to both excessive loading and unloading by producing pro-inflammatory mediators, suggesting that these cells are actively involved in the development of tendon degeneration. The transcription factor scleraxis (Scx) is required for the development of force-transmitting tendon during development and for mechanically stimulated tenogenesis of stem cells, but its function in adult tenocytes is less well-defined. The aim of this study was to further define the role of Scx in mediating the adult tenocyte mechanoresponse.
resultsEquine tenocytes exposed to siRNA targeting Scx or a control siRNA were maintained under cyclic mechanical strain before being submitted for RNA-seq analysis. Focal adhesions and extracellular matrix-receptor interaction were among the top gene networks downregulated in Scx knockdown tenocytes. Correspondingly, tenocytes exposed to Scx siRNA were significantly softer, with longer vinculin-containing focal adhesions, and an impaired ability to migrate on soft surfaces. Other pathways affected by Scx knockdown included increased oxidative phosphorylation and diseases caused by endoplasmic reticular stress, pointing to a larger role for Scx in maintaining tenocyte homeostasis.
conclusionsOur study identifies several novel roles for Scx in adult tenocytes, which suggest that Scx facilitates mechanosensing by regulating the expression of several mechanosensitive focal adhesion proteins. Furthermore, we identified a number of other pathways and targets affected by Scx knockdown that have the potential to elucidate the role that tenocytes may play in the development of degenerative tendinopathy.
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