Evidence map›Paper›PMID 30083841›Full record

ArticleIn vitro cellular & developmental biology. Animal2018

Unexpected dose response of HaCaT to UVB irradiation.

Rong-Shing Chang, Chi-Shuo Chen, Ching-Lung Huang, Chiu-Ting Chang, Yujia Cui, Wei-Ju Chung, Wun-Yi Shu, Chi-Shiun Chiang, Chun-Yu Chuang, Ian C Hsu

Abstract read
PubMed Publisher
In one paragraph

Article in In vitro cellular & developmental biology. Animal, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.2field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Rong-Shing ChangDepartment of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, 101, Section2, Kuang-Fu Road, Hsinchu, 30013, Taiwan.
Chi-Shuo ChenDepartment of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, 101, Section2, Kuang-Fu Road, Hsinchu, 30013, Taiwan.
Ching-Lung HuangDepartment of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, 101, Section2, Kuang-Fu Road, Hsinchu, 30013, Taiwan.
Chiu-Ting ChangDepartment of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, 101, Section2, Kuang-Fu Road, Hsinchu, 30013, Taiwan.
Yujia CuiDepartment of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, 101, Section2, Kuang-Fu Road, Hsinchu, 30013, Taiwan.
Wei-Ju Chung
Wun-Yi ShuInstitute of Statistics, National Tsing Hua University, Hsinchu, Taiwan.
Chi-Shiun ChiangDepartment of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, 101, Section2, Kuang-Fu Road, Hsinchu, 30013, Taiwan.
Chun-Yu ChuangDepartment of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, 101, Section2, Kuang-Fu Road, Hsinchu, 30013, Taiwan.
Ian C HsuDepartment of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, 101, Section2, Kuang-Fu Road, Hsinchu, 30013, Taiwan. ichsu@mx.nthu.edu.tw.ORCID http://orcid.org/0000-0003-2782-9997
National Tsing Hua University · TW

Funding

Ministry of Science and Technology, Taiwan MOST 104-2112-M-007-016
6 · The paper itself

Abstract

Application of high-dosage UVB irradiation in phototherapeutic dermatological treatments present health concerns attributed to UV-exposure. In assessing UV-induced photobiological damage, we investigated dose-dependent effects of UVB irradiation on human keratinocyte cells (HaCaT). Our study implemented survival and apoptosis assays and revealed an unexpected dose response wherein higher UVB-dosage induced higher viability. Established inhibitors, such as AKT- (LY294002), PKC- (Gö6976, and Rottlerin), ERK- (PD98059), P38 MAPK- (SB203580), and JNK- (SP600125), were assessed to investigate UV-induced apoptotic pathways. Despite unobvious contributions of known signaling pathways in dose-response mediation, microarray analysis identified transcriptional expression of UVB-response genes related to the respiratory-chain. Observed correlation of ROS-production with UVB irradiation potentiated ROS as the underlying mechanism for observed dose responses. Inability of established pathways to explain such responses suggests the complex nature underlying UVB-phototherapy response.

Indexed as

Ultraviolet RaysAcetophenonesApoptosisBenzopyransCell CycleCell LineCell SurvivalDose-Response Relationship, RadiationElectron TransportFlavonoidsGene Expression ProfilingHumansKeratinocytesNitric OxidePhosphatidylinositol 3-KinasesPoly(ADP-ribose) Polymerases2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-oneAcetophenonesBenzopyransFlavonoidsNitric OxidePhosphatidylinositol 3-KinasesPoly(ADP-ribose) PolymerasesProto-Oncogene Proteins c-aktReactive Oxygen SpeciesrottlerinSuperoxidesApoptosisPhototherapyProliferationROS, reactive oxygen speciesUVB, ultraviolet B

Identifiers

PMID30083841
OpenAlexW2887507903

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.