Metabolic engineering using iterative self-cloning to improve lipid productivity in Coccomyxa.

Yuki Kasai, Takuya Tsukahara, Fukiko Ikeda, Yoko Ide, Shigeaki Harayama

Open access · goldAbstract read

In one paragraph

Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it

0cells of the map it votes in

2citing papers in PubMed

0.6field-weighted citation impact, top 36% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 29 citations in OpenAlex.

Development of a CRISPR/Cas9-mediated gene-editing method to isolate a mutant of the unicellular green alga Parachlorella kessleri strain NIES-2152 with improved lipid productivity.Biotechnology for biofuels and bioproducts · 2024
Article
Bioengineering of Microalgae: Recent Advances, Perspectives, and Regulatory Challenges for Industrial Application.Frontiers in bioengineering and biotechnology · 2020
Review

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Yuki KasaiDepartment of Biological Sciences, Faculty of Science and Engineering, Chuo University, Bunkyo-ku, Tokyo, 112-8551, Japan. ykasai@kc.chuo-u.ac.jp.

Takuya TsukaharaDepartment of Biological Sciences, Faculty of Science and Engineering, Chuo University, Bunkyo-ku, Tokyo, 112-8551, Japan.

Fukiko IkedaDepartment of Biological Sciences, Faculty of Science and Engineering, Chuo University, Bunkyo-ku, Tokyo, 112-8551, Japan.

Yoko IdeDepartment of Biological Sciences, Faculty of Science and Engineering, Chuo University, Bunkyo-ku, Tokyo, 112-8551, Japan.

Shigeaki HarayamaDepartment of Biological Sciences, Faculty of Science and Engineering, Chuo University, Bunkyo-ku, Tokyo, 112-8551, Japan.

Chuo University · JP

Funding

New Energy and Industrial Technology Development Organization (NEDO) P11502725-0

6 · The paper itself

Abstract

We previously developed a self-cloning system that introduces cDNA of the uridine monophosphate synthase gene (cUMPS) of Coccomyxa sp. strain Obi as a selectable marker into uracil-auxotrophic mutants (Ura

Indexed as

ChlorophytaDNA, ComplementaryGenetic VectorsIntegrasesMetabolic EngineeringMicroalgaeMultienzyme ComplexesOrotate PhosphoribosyltransferaseOrotidine-5'-Phosphate DecarboxylasePlants, Genetically ModifiedRecombination, GeneticCre recombinaseDNA, ComplementaryIntegrasesMultienzyme ComplexesOrotate PhosphoribosyltransferaseOrotidine-5'-Phosphate Decarboxylaseuridine 5'-monophosphate synthase

Identifiers

PMID30082815

DOI10.1038/s41598-018-30254-7

PMCPMC6078956

OpenAlexW2887927437

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.