ArticleScientific reports2018
PLVAP and GKN3 Are Two Critical Host Cell Receptors Which Facilitate Japanese Encephalitis Virus Entry Into Neurons.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 46 citations in OpenAlex.
- Cellular receptors and host factors mediating orthoflavivirus entry.Virology journal · 2026Review
- Transcriptomic landscape of pseudorabies virus-induced encephalitis reveals key lncRNAs involved in host-neurotropic virus interactions.Veterinary research · 2025Article
- Mechanisms and Research Methods of Protein Modification in Virus Entry.Applied biochemistry and biotechnology · 2025Review
- Insights into mosquito-borne arbovirus receptors.Cell insight · 2024Review
- Proteome modulation triggers potent antiviral response in Japanese Encephalitis Virus infected human macrophages.Archives of microbiology · 2024Article
- Article
- Transcriptome analysis of the aged SAMP8 mouse model of Alzheimer's disease reveals novel molecular targets of formononetin protection.Frontiers in pharmacology · 2024Article
- Integrated clinical and metabolomic analysis identifies molecular signatures, biomarkers, and therapeutic targets in primary angle closure glaucoma.Frontiers in molecular biosciences · 2024Article
- Vector-Transmitted Flaviviruses: An Antiviral Molecules Overview.Microorganisms · 2023Review
- Virus-host Interactions in Early Japanese Encephalitis Virus Infection.Virus research · 2023Review
- The role of PLVAP in endothelial cells.Cell and tissue research · 2023Review
- Review
- Japanese Encephalitis Virus-Infected Cells.Sub-cellular biochemistry · 2023Article
- CXCL12 defines lung endothelial heterogeneity and promotes distal vascular growth.Development (Cambridge, England) · 2022Article
- Japanese encephalitis viral infection modulates proinflammatory cyto/chemokine profile in primary astrocyte and cell line of astrocytic origin.Metabolic brain disease · 2022Article
- Neuroinflammation in neurodegenerationFrontiers in immunology · 2022Review
- Article
- Pathogenesis and virulence of flavivirus infections.Virulence · 2021Review
- In Vitro Infection Dynamics of Japanese Encephalitis Virus in Established Porcine Cell Lines.Pathogens (Basel, Switzerland) · 2021Article
- Viral Membrane Fusion Proteins and RNA Sorting Mechanisms for the Molecular Delivery by Exosomes.Cells · 2021Review
Corrections and comments
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Authors and funding
9 authors at 5 institutions in 1 country.
Funding
Abstract
Japanese Encephalitis Virus (JEV), a globally important pathogen, belongs to the family Flaviviridae, is transmitted between vertebrate hosts by mosquitoes, principally by Culex tritaeniorhynchus. The E-glycoprotein of the virus mediates its attachment to the host cell receptors. In this study, we cloned and purified JEV E-glycoprotein in pET28a vector using E. coli BL21 (DE3) cells. A pull down assay was performed using plasma membrane fraction of BALB/c mouse brain and E-glycoprotein as a bait protein. 2-Dimensional Gel Electrophoresis based separation of the interacting proteins was analyzed by mass spectrometry. Among all the identified partners of E-glycoprotein, PLVAP (Plasmalemma vesicle associated protein) and GKN3 (Gastrokine3) showed significant up-regulation in both JEV infected mouse brain and neuro2a cells. In-silico studies also predicted significant interaction of these receptors with E-glycoprotein. Additionally, overexperssion and silencing of these receptors resulted in increase and reduction in viral load respectively, suggesting them as two critical cellular receptors governing JEV entry and propagation in neurons. In support, we observed significant expression of PLVAP but not GKN3 in post-mortem autopsied human brain tissue. Our results establish two novel receptor proteins in neurons in case of JEV infection, thus providing potential targets for antiviral research.
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