ArticleScientific reports2018
High doses of CRISPR/Cas9 ribonucleoprotein efficiently induce gene knockout with low mosaicism in the hydrozoan Clytia hemisphaerica through microhomology-mediated deletion.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 46 citations in OpenAlex.
- Problems With GO Enrichment as an Endpoint for Ecological Omics Interpretation.Molecular ecology · 2026Article
- Precise, predictable genome integrations by deep-learning-assisted design of microhomology-based templates.Nature biotechnology · 2026Article
- The jellyfish Clytia hemisphaerica.Nature methods · 2026Article
- A genetically tractable non-vertebrate system to study complete camera-type eye regeneration.Nature communications · 2025Article
- Genetically encoded affinity reagents are a toolkit for visualizing and manipulating endogenous protein function in vivo.Nature communications · 2025Article
- Microinjection, gene knockdown, and CRISPR-mediated gene knock-in in the hard coral, Astrangia poculata.EvoDevo · 2025Article
- Jellyfish for the study of nervous system evolution and function.Current opinion in neurobiology · 2024Review
- Clustered Regularly Interspaced Short Palindromic Repeat/CRISPR-Associated Protein and Its Utility All at Sea: Status, Challenges, and Prospects.Microorganisms · 2024Review
- Epithelial wound healing in Clytia hemisphaerica provides insights into extracellular ATP signaling mechanisms and P2XR evolution.Scientific reports · 2023Article
- Conserved meiotic mechanisms in the cnidarianScience advances · 2023Article
- Endogenous tagging of multiple cellular components in the sea anemoneProceedings of the National Academy of Sciences of the United States of America · 2023Article
- siRNA-mediated gene knockdown via electroporation in hydrozoan jellyfish embryos.Scientific reports · 2022Article
- Emerging Cnidarian Models for the Study of Epithelial Polarity.Frontiers in cell and developmental biology · 2022Review
- Article
- Review
- Article
- Pattern regulation in a regenerating jellyfish.eLife · 2020Article
- A G protein-coupled receptor mediates neuropeptide-induced oocyte maturation in the jellyfish Clytia.PLoS biology · 2020Article
- The PI3K subunits, P110α and P110β are potential targets for overcoming P-gp and BCRP-mediated MDR in cancer.Molecular cancer · 2020Article
- Function and Distribution of the Wamide Neuropeptide Superfamily in Metazoans.Frontiers in endocrinology · 2020Review
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Targeted mutagenesis using CRISPR/Cas9 technology has been shown to be a powerful approach to examine gene function in diverse metazoan species. One common drawback is that mixed genotypes, and thus variable phenotypes, arise in the F0 generation because incorrect DNA repair produces different mutations amongst cells of the developing embryo. We report here an effective method for gene knockout (KO) in the hydrozoan Clytia hemisphaerica, by injection into the egg of Cas9/sgRNA ribonucleoprotein complex (RNP). Expected phenotypes were observed in the F0 generation when targeting endogenous GFP genes, which abolished fluorescence in embryos, or CheRfx123 (that codes for a conserved master transcriptional regulator for ciliogenesis) which caused sperm motility defects. When high concentrations of Cas9 RNP were used, the mutations in target genes at F0 polyp or jellyfish stages were not random but consisted predominantly of one or two specific deletions between pairs of short microhomologies flanking the cleavage site. Such microhomology-mediated (MM) deletion is most likely caused by microhomology-mediated end-joining (MMEJ), which may be favoured in early stage embryos. This finding makes it very easy to isolate uniform, largely non-mosaic mutants with predictable genotypes in the F0 generation in Clytia, allowing rapid and reliable phenotype assessment.
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