ArticleBMC research notes2018
A novel technique for isolating DNA from Tempus™ blood RNA tubes after RNA isolation.
Article in BMC research notes, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed, 6 citations in OpenAlex.
- Comparison of RNA Extraction Method for Human Whole Blood: Assessing the Quality, Quantity, and Impact of RBC Lysis Across TRIzol, Invitrogen, and Qiagen Systems.Drug testing and analysis · 2026Article
- Blood RNA biomarkers and a point-of-care elastase assay for detecting host immune activation in suspected sepsis: Trajectory matters.PloS one · 2025Article
- Characteristics of RNA Stabilizer RNApro for Peripheral Blood Collection.Diagnostics (Basel, Switzerland) · 2024Article
- Evaluation of quantitative biomarkers of aging in human PBMCs.Frontiers in aging · 2023Article
Corrections and comments
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Authors and funding
4 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveWe use Tempus blood RNA tubes (Applied Biosystems) during health assessments of American moose (Alces alces spp.) as a minimally invasive means to obtain RNA. Here we describe a novel protocol to additionally isolate high-quality DNA from the supernatant remaining after the RNA isolation methodology. Metrics used to qualify DNA quality included measuring the concentration, obtaining a DNA integrity number from a genomic DNA ScreenTape assay (Agilent), and running the isolated DNA on an agarose gel.
resultsOf the 23 samples analyzed, the average DNA concentration was 121 ng/µl (range 4-337 ng/µl) and a genomic DNA ScreenTape assay of seven samples indicated high DNA integrity values for 6 of the 7 samples (range 9.1-9.4 out of 10). Of the DNA sent for genotyping by sequencing, all proved to be of sufficient integrity to yield high-quality next-generation sequence results. We recommend this simple procedure to maximize the yield of both RNA and DNA from blood samples.
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Registered trials
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