Evidence map›Paper›PMID 30081661›Full record

ArticleCurrent eye research2018

Comparison of Collection Methods for the Measure of Human Meibum and Tear Film-Derived Lipids Using Mass Spectrometry.

William Ngo, Jianzhong Chen, Shyam Panthi, Kelly K Nichols, Jason J Nichols

Open access · bronzeAbstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Current eye research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed, 1 pooled it
2.3field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 1 synthesis or guideline pooled it, 22 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Meibomian Gland Shortening Is Associated With Altered Meibum Composition.Investigative ophthalmology & visual science · 2024
    Article
  4. Review
  5. Article
  6. Article
  7. Article
  8. Article
  9. Mass Spectrometric Analysis of Meibomian Gland Lipids.Methods in molecular biology (Clifton, N.J.) · 2021
    Article
  10. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

William Ngoa School of Optometry, Department of Optometry & Vision Science , The University of Alabama at Birmingham , Birmingham , AL , USA.
Jianzhong Chena School of Optometry, Department of Optometry & Vision Science , The University of Alabama at Birmingham , Birmingham , AL , USA.
Shyam Panthia School of Optometry, Department of Optometry & Vision Science , The University of Alabama at Birmingham , Birmingham , AL , USA.
Kelly K Nicholsa School of Optometry, Department of Optometry & Vision Science , The University of Alabama at Birmingham , Birmingham , AL , USA.
Jason J Nicholsa School of Optometry, Department of Optometry & Vision Science , The University of Alabama at Birmingham , Birmingham , AL , USA.
University of Alabama at Birmingham · US

Funding

The role of (O-acyl)-omega-hydroxy fatty acids in human lipid layer structure and function in health and meibomian gland dysfunction.R01EY026947 · NEI · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI NICHOLS, JASON JAY · 2016 to 2020
$1.8M
NEI NIH HHS R01 EY026947
6 · The paper itself

Abstract

Purpose/aim: To assess the effectiveness of polytetrafluoroethylene (PTFE) tubes in the collection of human tears and meibum. MATERIALS AND

methodsThis was a prospective study that enrolled 10 healthy human subjects. Both the tear film and meibum were sampled using PTFE tubes in the right eye of all subjects. In the left eyes, either 5-µL or 1-µL glass microcapillary tubes were used to collect tears, and 0.5-µL glass microcapillary tubes were used to collect meibum. The lipids from the samples were extracted and analyzed using mass spectrometry (SCIEX TripleTOF 5600, Framingham, MA, USA). The absolute peak intensities of the omega-acyl hydroxy fatty acids (OAHFA), cholesterol esters (CE), and wax esters (WE) obtained for both methods were summed and compared between collection methods.

resultsA total of 10 subjects completed the study (five female, mean age: 35.7 ± 7.9 years). Using the mass spectrometer output, the median (first quartile, third quartile) summed intensity units of OAHFA, CE, and WE collected associated with tears using PTFE were 516 (125, 1315), 7946 (2571, 19,915), and 38,892 (139,630, 174,082), all of which were significantly higher (all p ≤ 0.04) than those collected from glass microcapillaries (91 (41, 408), 2463 (1389, 6042), and 11,109 (7465, 37,371), respectively). The median summed intensity units of OAHFA, CE, and WE associated with meibum (1958 (1417, 3502), 11,726 (8434, 87,691), and 84,771 (52,657, 206,050), respectively) using PTFE were not significantly different (all p ≥ 0.13) than those associated with glass microcapillaries (1502 (699, 4407), 10,781 (3287, 38,205), and 77,381 (26,590, 178,213), respectively).

conclusionsPTFE tubes, which are thought to be lipophilic, were associated with more measurable lipids from the tear film than glass microcapillaries. There was no difference between collection methods in lipid profiles when used with meibum.

Indexed as

GlassPolytetrafluoroethyleneAdultFemaleHumansLipidsMaleMass SpectrometryMeibomian GlandsMiddle AgedProspective StudiesSpecimen HandlingTearsLipidsPolytetrafluoroethylenedry eyelipidsmass spectrometrymeibomian glandTear film

Identifiers

PMID30081661
PMCPMC7939036
OpenAlexW2886487194

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.