ArticleBrain sciences2018
New Insights into GFAP Negative Astrocytes in Calbindin D28k Immunoreactive Astrocytes.
Article in Brain sciences, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 18 citations in OpenAlex.
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- Astrocytes display ultrastructural alterations and heterogeneity in the hippocampus of aged APP-PS1 mice and human post-mortem brain samples.Journal of neuroinflammation · 2023Article
- Generation and Characterization of the First Murine Model of Alzheimer's Disease with Mutated AβPP Inserted in a BALB/c Background (C.B6/J-APPswe).Journal of Alzheimer's disease : JAD · 2023Article
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- The role of glia in the physiology and pharmacology of glucagon-like peptide-1: implications for obesity, diabetes, neurodegeneration and glaucoma.British journal of pharmacology · 2022Review
- Melanocortin receptor agonist NDP-α-MSH improves cognitive deficits and microgliosis but not amyloidosis in advanced stages of AD progression in 5XFAD and 3xTg mice.Frontiers in immunology · 2022Article
- Targeting CDK5 in Astrocytes Promotes Calcium Homeostasis Under Excitotoxic Conditions.Frontiers in cellular neuroscience · 2021Article
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- Regionally specified human pluripotent stem cell-derived astrocytes exhibit different molecular signatures and functional properties.Development (Cambridge, England) · 2019Article
- CB2R induces a protective response for epileptic seizure via the PI3K 110α-AKT signaling pathway.Experimental and therapeutic medicine · 2018Article
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Glial fibrillary acidic protein (GFAP) is commonly used as a specific marker for the identification of astrocytes. Nevertheless, it is known from the literature that astrocytes in situ in contrast to cultured astrocytes may feature lower levels of GFAP. In order to characterize the properties of GFAP in Calbindin D28k immunoreactive astrocytes, we use primary astrocyte cultures from cells of new-born mice. A double fluorescence immunocytochemical analysis reveals that GFAP in cultured Calbindin D28k astrocytes behaves differently depending on whether the medium contains foetal bovine serum (FBS) or not. The novelty in our study is, however, that a high percentage of Calbindin D28k cultured astrocytes in a medium with 10% FBS are GFAP negative. In addition, the study shows that Calbindin D28k astrocytes have (i) a different morphology and (ii) a higher concentration of Calbindin D28k in the nucleus than in the cytoplasm. The study provides new evidence that in order to fully understand the characteristics of astrocytes, astrocytes which are Calbindin D28k positive have to be investigated.
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