Evidence map›Paper›PMID 30081233›Full record

ArticleMolecular therapy. Nucleic acids2018

Foamy Virus Vectors Transduce Visceral Organs and Hippocampal Structures following In Vivo Delivery to Neonatal Mice.

John R Counsell, Rajvinder Karda, Juan Antinao Diaz, Louise Carey, Tatiana Wiktorowicz, Suzanne M K Buckley, Shima Ameri, Joanne Ng, Julien Baruteau, Filipa Almeida and 9 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular therapy. Nucleic acids, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.7field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 9 citations in OpenAlex.

  1. Review
  2. Recent Advances in In Vivo Somatic Cell Gene Modification in Newborn Pups.International journal of molecular sciences · 2023
    Review
  3. Article
  4. Article
  5. Red Light Optogenetics in Neuroscience.Frontiers in cellular neuroscience · 2021
    Review
  6. Gene therapy for inherited metabolic diseases.Journal of mother and child · 2020
    Review
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors at 3 institutions in 3 countries.

John R CounsellGene Transfer Technology Group, EGA Institute for Women's Health, University College London, London WC1E 6HX, UK; Dubowitz Neuromuscular Centre, Molecular Neurosciences Section, Developmental Neurosciences Programme, UCL Great Ormond Street Institute of Child Health, 30 Guilford Street, London WC1N 1EH, UK; NIHR Great Ormond Street Hospital Biomedical Research Centre, 30 Guilford Street, London WC1N 1EH, UK.
Rajvinder KardaGene Transfer Technology Group, EGA Institute for Women's Health, University College London, London WC1E 6HX, UK.
Juan Antinao DiazGene Transfer Technology Group, EGA Institute for Women's Health, University College London, London WC1E 6HX, UK.
Louise CareyGene Transfer Technology Group, EGA Institute for Women's Health, University College London, London WC1E 6HX, UK.
Tatiana WiktorowiczUniversität Würzburg, Institut für Virologie und Immunbiologie, Versbacher Str. 7, 97078 Würzburg, Germany.
Suzanne M K BuckleyGene Transfer Technology Group, EGA Institute for Women's Health, University College London, London WC1E 6HX, UK.
Shima AmeriGene Transfer Technology Group, EGA Institute for Women's Health, University College London, London WC1E 6HX, UK.
Joanne NgGene Transfer Technology Group, EGA Institute for Women's Health, University College London, London WC1E 6HX, UK.
Julien BaruteauGene Transfer Technology Group, EGA Institute for Women's Health, University College London, London WC1E 6HX, UK.
Filipa AlmeidaReta Lila Weston Institute and Department of Molecular Neuroscience, UCL Institute of Neurology, London WC1N 3BG, UK.
Rohan de SilvaReta Lila Weston Institute and Department of Molecular Neuroscience, UCL Institute of Neurology, London WC1N 3BG, UK.
Roberto SimoneReta Lila Weston Institute and Department of Molecular Neuroscience, UCL Institute of Neurology, London WC1N 3BG, UK.
Eleonora LugaràDepartment of Clinical and Experimental Epilepsy, Queen Square House, UCL Institute of Neurology, London WC1N 3BG, UK.
Gabriele LignaniDepartment of Clinical and Experimental Epilepsy, Queen Square House, UCL Institute of Neurology, London WC1N 3BG, UK.
Dirk LindemannUniversität Würzburg, Institut für Virologie und Immunbiologie, Versbacher Str. 7, 97078 Würzburg, Germany; Institute of Virology, Technische Universität Dresden, Dresden, Germany; Center for Regenerative Therapies Dresden (CRTD), Technische Universität Dresden, Dresden, Germany.
Axel RethwilmUniversität Würzburg, Institut für Virologie und Immunbiologie, Versbacher Str. 7, 97078 Würzburg, Germany.
Ahad A RahimDepartment of Pharmacology, UCL School of Pharmacy, University College London, London WC1N 1AX, UK.
Simon N WaddingtonGene Transfer Technology Group, EGA Institute for Women's Health, University College London, London WC1E 6HX, UK; Wits/SAMRC Antiviral Gene Therapy Research Unit, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa. Electronic address: s.waddington@ucl.ac.uk.
Steven J HoweGene Transfer Technology Group, EGA Institute for Women's Health, University College London, London WC1E 6HX, UK.
University College London · GBUniversity of Würzburg · DENational Hospital for Neurology and Neurosurgery · GB

Funding

Medical Research Council G0501560Medical Research Council G1000709Medical Research Council MC_PC_14118Medical Research Council MR/K02342X/1Medical Research Council MR/N026101/1Medical Research Council MR/P026494/1Medical Research Council MR/R015325/1Parkinson's UK K-1212Wellcome Trust 210774/Z/18/Z
6 · The paper itself

Abstract

Viral vectors are rapidly being developed for a range of applications in research and gene therapy. Prototype foamy virus (PFV) vectors have been described for gene therapy, although their use has mainly been restricted to ex vivo stem cell modification. Here we report direct in vivo transgene delivery with PFV vectors carrying reporter gene constructs. In our investigations, systemic PFV vector delivery to neonatal mice gave transgene expression in the heart, xiphisternum, liver, pancreas, and gut, whereas intracranial administration produced brain expression until animals were euthanized 49 days post-transduction. Immunostaining and confocal microscopy analysis of injected brains showed that transgene expression was highly localized to hippocampal architecture despite vector delivery being administered to the lateral ventricle. This was compared with intracranial biodistribution of lentiviral vectors and adeno-associated virus vectors, which gave a broad, non-specific spread through the neonatal mouse brain without regional localization, even when administered at lower copy numbers. Our work demonstrates that PFV can be used for neonatal gene delivery with an intracranial expression profile that localizes to hippocampal neurons, potentially because of the mitotic status of the targeted cells, which could be of use for research applications and gene therapy of neurological disorders.

Indexed as

bioimagingfoamy virusgene therapyhippocampusspumavirusvector tropismviral vector

Identifiers

PMID30081233
PMCPMC6082918
OpenAlexW2886714605

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.