ArticlePloS one2018
Processed eggshell membrane powder regulates cellular functions and increase MMP-activity important in early wound healing processes.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Eggshell membrane-based biomaterials for tissue regeneration: a systematic review of preclinical evidence.Frontiers in bioengineering and biotechnology · 2026Pooled it
- Electrospun polyurethane-eggshell membrane hybrid nanofibrous scaffolds for enhanced fibroblast adhesion and growth in skin tissue engineering.Scientific reports · 2026Article
- The Promise of 3D Biomaterial Bioprinting for Wound-Healing and Skin Tissue Restoration.Life (Basel, Switzerland) · 2026Review
- Design and optimization of a shearing grinding device for efficient separation of eggshell and eggshell membrane.Frontiers in bioengineering and biotechnology · 2026Article
- Adipose-derived stem cell exosomes: mechanisms and therapeutic potentials in wound healing.Biomarker research · 2025Review
- Development of a Multilayer Film Including the Soluble Eggshell Membrane Fraction for the Treatment of Oral Mucosa Lesions.Pharmaceutics · 2024Article
- A comparison of several separation processes for eggshell membrane powder as a natural biomaterial for skin regeneration.Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI) · 2024Article
- Eggshell membrane and green seaweed (Ulva lactuca) micronized powders for in vivo diabetic wound healing in albino rats: a comparative study.Journal of pharmaceutical health care and sciences · 2024Article
- Article
- Combined Pulsed Magnetic Field and Radiofrequency Electromagnetic Field Enhances MMP-9, Collagen-4, VEGF Synthesis to Improve Wound Healing Via Hif-1α/eNOS Pathway.Aesthetic plastic surgery · 2023Article
- Eggshell Membrane as a Biomaterial for Bone Regeneration.Polymers · 2023Review
- Advances in eggshell membrane separation and solubilization technologies.Frontiers in veterinary science · 2023Review
- Eggshell membrane treatment for the healing of superficial open wounds in miceBiomedica : revista del Instituto Nacional de Salud · 2022Article
- High Efficiency In Vitro Wound Healing ofJournal of fungi (Basel, Switzerland) · 2021Article
- Avian Eggshell Membrane as a Novel Biomaterial: A Review.Foods (Basel, Switzerland) · 2021Review
- Processed Eggshell Membrane Powder Is a Promising Biomaterial for Use in Tissue Engineering.International journal of molecular sciences · 2020Article
- Article
- State-of-the-Art of Eggshell Waste in Materials Science: Recent Advances in Catalysis, Pharmaceutical Applications, and Mechanochemistry.Frontiers in bioengineering and biotechnology · 2020Review
- [Changes of lymphatic vessel density in lung adenocarcinoma in situ, minimally invasive adenocarcinoma, and invasive adenocarcinoma and the regulatory factors].Nan fang yi ke da xue xue bao = Journal of Southern Medical University · 2018Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Avian eggshell membrane (ESM) is a natural biomaterial that has been used as an alternative natural bandage to cure wounds, and is available in large quantities from egg industries. We have previously demonstrated that processed eggshell membrane powder (PEP), aiming to be used in a low cost wound healing product, possesses anti-inflammatory properties. In this study, we further investigated effects of PEP on MMP activities in vitro (a dermal fibroblast cell culture system) and in vivo (a mouse skin wound healing model). Three days incubation with PEP in cell culture led to rearrangement of the actin-cytoskeleton and vinculin in focal adhesions and increased syndecan-4 shedding. In addition, we observed increased matrix metalloproteinase type 2 (MMP-2) enzyme activation, without effects on protein levels of MMP-2 or its regulators (membrane type 1 (MT1)-MMP and tissue inhibitor of matrix metalloproteinase type 2 (TIMP-2). Longer incubation (10 days) led to increased protein levels of MMP-2 and its regulators. We also observed an increased alpha-smooth muscle actin (α-SMA) production, suggesting an effect of PEP on myofibroblast differentiation. In vivo, using the mouse skin wound healing model, PEP treatment (3 days) increased MMP activity at the wound edges, along with increased MMP-2 and MMP-9 protein levels, and increased keratinocyte cell proliferation. Altogether, our data suggest PEP stimulates MMP activity, and with a positive effect on early cellular events during wound healing.
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