ArticleNature biotechnology2018
Genome editing of upstream open reading frames enables translational control in plants.
Article in Nature biotechnology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 173 papers.
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Who cites it
173 citing papers in PubMed, 351 citations in OpenAlex.
- OmniEdit: A unified CRISPR/Cas9 platform for precise genome engineering and strain optimization of theSynthetic and systems biotechnology · 2026Article
- Balancing tuber vitamin C improvement with trade-off side effect by fine-tuning regulation ofHorticulture research · 2026Article
- Natural and CRISPR/Cas9 Editing Variations of Dual-uORFs Synergistically Increase the Nutritional Value of Silage Maize Feed.Plant biotechnology journal · 2026Article
- Overlapping upstream ORFs repress translation and expand proteome diversity in Arabidopsis.bioRxiv : the preprint server for biology · 2026Article
- Unlocking the chemical potential of filamentous fungi using prime editing.Nature biotechnology · 2026Article
- Prime editing for precise genome engineering and modulation of fungal metabolism.Nature biotechnology · 2026Article
- Review
- CRISPR-Cas9-mediated uATG introduction in the 5'UTR of the Uox gene for hyperuricemia mouse models: implications for gout and metabolic disorders.Science China. Life sciences · 2026Article
- A Fluorescence-Based Transient Expression Assay for the Analysis of Upstream Open Reading Frames in Plants.Plant direct · 2026Article
- Targeted BDNF upregulation via upstream open reading frame disruption.Molecular therapy : the journal of the American Society of Gene Therapy · 2026Article
- OsCPK9-mediated Thr105 phosphorylation activates OsCATC to enhance drought tolerance in rice.Journal of advanced research · 2026Article
- Review
- Multiplex gene editing drives revolution in crop breeding: overlaid editing of multiple genes and customization of complex traits.Advanced biotechnology · 2026Review
- Targeted Gene Modification ofInternational journal of molecular sciences · 2026Article
- Effects of OsNRT2.3b transgenosis on lettuce antioxidant capacity and nitrogen metabolism under low nitrogen.PloS one · 2026Article
- Translational control in plants: from basic mechanisms to environmental and developmental responses.The Plant journal : for cell and molecular biology · 2026Review
- Precision engineering of upstream open reading frames for translational regulation and crop improvement.Frontiers in genome editing · 2026Review
- CRISPR/Cas9-mediated uORF engineering enhances tanshinone biosynthesis inHorticulture research · 2026Article
- From reference-genome prediction to causal generalization: sequence-to-function models for plant regulatory genomics.Frontiers in plant science · 2026Review
- CRISPR-Cas technologies for precision genome editing in plants: advances, applications, and future perspectives.Frontiers in plant science · 2026Review
113 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Translational regulation by upstream open reading frames (uORFs) is becoming established as a general mechanism for controlling the amount of protein that is synthesized from downstream primary ORFs (pORFs). We found that genome editing of endogenous uORFs in plants enabled the modulation of translation of mRNAs from four pORFs that are involved in either development or antioxidant biosynthesis. A single-guide RNA that targeted the region harboring a uORF initiation codon can produce multiple mutations. Following uORF editing, we observed varying amounts of mRNA translation in four pORFs. Notably, editing the uORF of LsGGP2, which encodes a key enzyme in vitamin C biosynthesis in lettuce, not only increased oxidation stress tolerance, but also increased ascorbate content by ∼150%. These data indicate that editing plant uORFs provides a generalizable, efficient method for manipulating translation of mRNA that could be applied to dissect biological mechanisms and improve crops.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.