ArticleeLife2018
Mitotic progression, arrest, exit or death relies on centromere structural integrity, rather than de novo transcription.
Article in eLife, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 22 citations in OpenAlex.
- Trial
- Global stabilization of the transcriptome in mitotic cells.The EMBO journal · 2026Article
- Article
- Natural degradation of RNA polymerase II is essential for oocyte chromatin reorganization and maternal-to-zygotic transition.Nature communications · 2025Article
- Polo-like kinase 1 maintains transcription and chromosomal accessibility during mitosis.Molecular biology of the cell · 2025Article
- Transcriptional repression across mitosis: mechanisms and functions.Biochemical Society transactions · 2024Review
- Rac1 promotes kidney collecting duct repair by mechanically coupling cell morphology to mitotic entry.Science advances · 2024Article
- Micronuclei from misaligned chromosomes that satisfy the spindle assembly checkpoint in cancer cells.Current biology : CB · 2022Article
- An emerging role of transcription in chromosome segregation: Ongoing centromeric transcription maintains centromeric cohesion.BioEssays : news and reviews in molecular, cellular and developmental biology · 2022Review
- Kinetochore assembly throughout the cell cycle.Seminars in cell & developmental biology · 2021Article
- Centromeric transcription maintains centromeric cohesion in human cells.The Journal of cell biology · 2021Article
- Novel insights into extrachromosomal DNA: redefining the onco-drivers of tumor progression.Journal of experimental & clinical cancer research : CR · 2020Review
- Functioning mechanisms of Shugoshin-1 in centromeric cohesion during mitosis.Essays in biochemistry · 2020Review
- Cohesin Removal Reprograms Gene Expression upon Mitotic Entry.Molecular cell · 2020Article
- The Emerging Role of ncRNAs and RNA-Binding Proteins in Mitotic Apparatus Formation.Non-coding RNA · 2020Review
- Article
- Mammalian cell growth dynamics in mitosis.eLife · 2019Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
Abstract
Recent studies have challenged the prevailing dogma that transcription is repressed during mitosis. Transcription was also proposed to sustain a robust spindle assembly checkpoint (SAC) response. Here, we used live-cell imaging of human cells, RNA-seq and qPCR to investigate the requirement for de novo transcription during mitosis. Under conditions of persistently unattached kinetochores, transcription inhibition with actinomycin D, or treatment with other DNA-intercalating drugs, delocalized the chromosomal passenger complex (CPC) protein Aurora B from centromeres, compromising SAC signaling and cell fate. However, we were unable to detect significant changes in mitotic transcript levels. Moreover, inhibition of transcription independently of DNA intercalation had no effect on Aurora B centromeric localization, SAC response, mitotic progression, exit or death. Mechanistically, we show that DNA intercalating agents reduce the interaction of the CPC with nucleosomes. Thus, mitotic progression, arrest, exit or death is determined by centromere structural integrity, rather than de novo transcription.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.