ArticleMicrobial genomics2018
Extended-spectrum β-lactamase-encoding genes are spreading on a wide range of Escherichia coli plasmids existing prior to the use of third-generation cephalosporins.
Article in Microbial genomics, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed, 43 citations in OpenAlex.
- Tracking Extended-Spectrum β-Lactamase-ProducingAntibiotics (Basel, Switzerland) · 2026Article
- Population-based Study of Carbapenem-resistantOpen forum infectious diseases · 2026Article
- Integrated Phenotypic and Genomic Characterization of Cefotaxime/Clavulanic Acid Inhibitor-Positive Multidrug-ResistantAnimals : an open access journal from MDPI · 2026Article
- Evidence of ESBL plasmid transfer and selective persistence of multiple host-associatedApplied and environmental microbiology · 2025Article
- Systematic functional assessment of anti-phage systems in their native host.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025Article
- Extended-spectrum beta-lactamases: definition, history, an update on their genetic environment and detection methods.Journal of medical microbiology · 2025Review
- Exogenous plasmid capture to characterize tetracycline-resistance plasmids in sprouts obtained from retail in Germany.Frontiers in microbiology · 2025Article
- Genomic insights into antimicrobial resistance and virulence ofFrontiers in microbiology · 2025Article
- Applying rearrangement distances to enable plasmid epidemiology with pling.Microbial genomics · 2024Article
- The plasmidome associated with Gram-negative bloodstream infections: A large-scale observational study using complete plasmid assemblies.Nature communications · 2024Article
- ISMicrobiology spectrum · 2024Article
- Article
- Risk factors for antibiotic resistance and mortality in patients with bloodstream infection of Escherichia coli.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2022Article
- Antimicrobial Peptides: From Design to Clinical Application.Antibiotics (Basel, Switzerland) · 2022Review
- Deciphering the genetic network and programmed regulation of antimicrobial resistance in bacterial pathogens.Frontiers in cellular and infection microbiology · 2022Review
- Use of Nanopore Sequencing to Characterise the Genomic Architecture of Mobile Genetic Elements EncodingFrontiers in microbiology · 2022Article
- Genomic Characterization of a UropathogenicFrontiers in microbiology · 2022Article
- Genomic network analysis of environmental and livestock F-type plasmid populations.The ISME journal · 2021Article
- Phylum barrier andMicrobial genomics · 2021Article
- Laboratory Stock Variants of the Archetype Silver Resistance Plasmid pMG101 Demonstrate Plasmid Fusion, Loss of Transmissibility, and Transposition of TnFrontiers in microbiology · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
18 authors at 11 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To understand the evolutionary dynamics of extended-spectrum β-lactamase (ESBL)-encoding genes in Escherichia coli, we undertook a comparative genomic analysis of 116 whole plasmid sequences of human or animal origin isolated over a period spanning before and after the use of third-generation cephalosporins (3GCs) using a gene-sharing network approach. The plasmids included 82 conjugative, 22 mobilizable and 9 non-transferable plasmids and 3 P-like bacteriophages. ESBL-encoding genes were found on 64 conjugative, 6 mobilizable, 2 non-transferable plasmids and 2 P1-like bacteriophages, indicating that these last three types of mobile elements also play a role, albeit modest, in the diffusion of the ESBLs. The network analysis showed that the plasmids clustered according to their genome backbone type, but not by origin or period of isolation or by antibiotic-resistance type, including type of ESBL-encoding gene. There was no association between the type of plasmid and the phylogenetic history of the parental strains. Finer scale analysis of the more abundant clusters IncF and IncI1 showed that ESBL-encoding plasmids and plasmids isolated before the use of 3GCs had the same diversity and phylogenetic history, and that acquisition of ESBL-encoding genes had occurred during multiple independent events. Moreover, the blaCTX-M-15 gene, unlike other CTX-M genes, was inserted at a hot spot in a blaTEM-1-Tn2 transposon. These findings showed that ESBL-encoding genes have arrived on wide range of pre-existing plasmids and that the successful spread of blaCTX-M-15 seems to be favoured by the presence of well-adapted IncF plasmids that carry a Tn2-blaTEM-1 transposon.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.