ArticleACS nano2018
A Robust Liposomal Platform for Direct Colorimetric Detection of Sphingomyelinase Enzyme and Inhibitors.
Article in ACS nano, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 44 citations in OpenAlex.
- Article
- Gold Nanoparticles-Based Colorimetric Immunoassay of Carcinoembryonic Antigen with Metal-Organic Framework to Load Quinones for Catalytic Oxidation of Cysteine.Sensors (Basel, Switzerland) · 2024Article
- Colorimetric sensing for translational applications: from colorants to mechanisms.Chemical Society reviews · 2024Review
- Cysteine-Encapsulated Liposome for Investigating Biomolecular Interactions at Lipid Membranes.International journal of molecular sciences · 2022Article
- Noble Metal Nanoparticle Biosensors: From Fundamental Studies toward Point-of-Care Diagnostics.Accounts of chemical research · 2022Review
- High-throughput glycolytic inhibitor discovery targeting glioblastoma by graphite dots-assisted LDI mass spectrometry.Science advances · 2022Article
- Acid Sphingomyelinase, a Lysosomal and Secretory Phospholipase C, Is Key for Cellular Phospholipid Catabolism.International journal of molecular sciences · 2021Review
- Acid-Sphingomyelinase Triggered Fluorescently Labeled Sphingomyelin Containing Liposomes in Tumor Diagnosis after Radiation-Induced Stress.International journal of molecular sciences · 2021Article
- The effect of pH and transition metal ions on cysteine-assisted gold aggregation for a distinct colorimetric response.RSC advances · 2021Article
- Controlled Dendrimersome Nanoreactor System for Localized Hypochlorite-Induced Killing of Bacteria.ACS nano · 2020Article
- A rapid and highly sensitive biomarker detection platform based on a temperature-responsive liposome-linked immunosorbent assay.Scientific reports · 2020Article
- A High-Throughput Assay to Identify Allosteric Inhibitors of the PLC-γ Isozymes Operating at Membranes.Biochemistry · 2020Article
- Cationic liposomes for generic signal amplification strategies in bioassays.Analytical and bioanalytical chemistry · 2020Article
- Advances of Nano-Structured Extended-Release Local Anesthetics.Nanoscale research letters · 2020Review
- A colorimetric method for determination of the prostate specific antigen based on enzyme-free cascaded signal amplification via peptide-copper(II) nanoparticles.Mikrochimica acta · 2020Article
- Controlling Secretion in Artificial Cells with a Membrane AND Gate.ACS synthetic biology · 2019Article
- Nanomaterials-Based Colorimetric Immunoassays.Nanomaterials (Basel, Switzerland) · 2019Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
The enzyme sphingomyelinase (SMase) is an important biomarker for several diseases such as Niemann Pick's, atherosclerosis, multiple sclerosis, and HIV. We present a two-component colorimetric SMase activity assay that is more sensitive and much faster than currently available commercial assays. Herein, SMase-triggered release of cysteine from a sphingomyelin (SM)-based liposome formulation with 60 mol % cholesterol causes gold nanoparticle (AuNP) aggregation, enabling colorimetric detection of SMase activities as low as 0.02 mU/mL, corresponding to 1.4 pM concentration. While the lipid composition offers a stable, nonleaky liposome platform with minimal background signal, high specificity toward SMase avoids cross-reactivity of other similar phospholipases. Notably, use of an SM-based liposome formulation accurately mimics the natural in vivo substrate: the cell membrane. We studied the physical rearrangement process of the lipid membrane during SMase-mediated hydrolysis of SM to ceramide using small- and wide-angle X-ray scattering. A change in lipid phase from a liquid to gel state bilayer with increasing concentration of ceramide accounts for the observed increase in membrane permeability and consequent release of encapsulated cysteine. We further demonstrated the effectiveness of the sensor in colorimetric screening of small-molecule drug candidates, paving the way for the identification of novel SMase inhibitors in minutes. Taken together, the simplicity, speed, sensitivity, and naked-eye readout of this assay offer huge potential in point-of-care diagnostics and high-throughput drug screening.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.