ArticleInsect molecular biology2019
Site-specific transgenesis of the Drosophila melanogaster Y-chromosome using CRISPR/Cas9.
Article in Insect molecular biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 41 citations in OpenAlex.
- Genetic engineering for SIT application: a fruit fly-focused review.Insect science · 2026Review
- Neoclassical development of genetic sexing strains for insect pest and disease vector control.Insect science · 2026Review
- Synthetic biology approaches to generate temperature-sensitive alleles for the Sterile Insect Technique.Insect science · 2026Review
- Functional validation of a white pupae minimal gene construct in Ceratitis capitata (Diptera: Tephritidae).Insect science · 2026Article
- Generating cisgenic sexing strains in insect pests.Communications biology · 2026Article
- Mosquito sex separation using complementation of selectable traits and engineered neo-sex chromosomes.Nature communications · 2025Article
- Decoding and engineering temperature-sensitive lethality inProceedings of the National Academy of Sciences of the United States of America · 2025Article
- A Y chromosome-linked genome editor for efficient population suppression in the malaria vector Anopheles gambiae.Nature communications · 2025Article
- Targeting mosquito X-chromosomes reveals complex transmission dynamics of sex ratio distorting gene drives.Nature communications · 2024Article
- The Drosophila melanogaster Y-linked gene, WDY, is required for sperm to swim in the female reproductive tract.Communications biology · 2024Article
- Exploiting a Y chromosome-linked Cas9 for sex selection and gene drive.Nature communications · 2021Article
- Transcribed sex-specific markers on the Y chromosome of the oriental fruit fly, Bactrocera dorsalis.BMC genetics · 2020Article
- Genetic engineering of sex chromosomes for batch cultivation of non-transgenic, sex-sorted males.PLoS genetics · 2020Article
- Adequacy and sufficiency evaluation of existing EFSA guidelines for the molecular characterisation, environmental risk assessment and post-market environmental monitoring of genetically modified insects containing engineered gene drives.EFSA journal. European Food Safety Authority · 2020Article
- Advances and challenges in genetic technologies to produce single-sex litters.PLoS genetics · 2020Review
- Dissecting Fertility Functions ofGenetics · 2020Article
- A fly model establishes distinct mechanisms for synthetic CRISPR/Cas9 sex distorters.PLoS genetics · 2020Article
- Progress towards engineering gene drives for population control.The Journal of experimental biology · 2020Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 2 countries.
Funding
Abstract
Despite the importance of Y-chromosomes in evolution and sex determination, their heterochromatic, repeat-rich nature makes them difficult to sequence (due, in part, to ambiguities in sequence alignment and assembly) and to genetically manipulate. Therefore, they generally remain poorly understood. For example, the Drosophila melanogaster Y-chromosome, one of the most extensively studied Y-chromosomes, is widely heterochromatic and composed mainly of highly repetitive sequences, with only a handful of expressed genes scattered throughout its length. Efforts to insert transgenes on this chromosome have thus far relied on either random insertion of transposons (sometimes harbouring 'landing sites' for subsequent integrations) with limited success or on chromosomal translocations, thereby limiting the types of Y-chromosome-related questions that could be explored. Here, we describe a versatile approach to site-specifically insert transgenes on the Y-chromosome in D. melanogaster via CRISPR/Cas9-mediated homology-directed repair. We demonstrate the ability to insert, and detect expression from, fluorescently marked transgenes at two specific locations on the Y-chromosome, and we utilize these marked Y-chromosomes to detect and quantify rare chromosomal nondisjunction effects. Finally, we discuss how this Y-docking technique could be adapted to other insects to aid in the development of genetic control technologies for the management of insect disease vectors and pests.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.