ArticleThe Journal of reproduction and development2018
Effects of recombinant OVGP1 protein on in vitro bovine embryo development.
Article in The Journal of reproduction and development, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 32 citations in OpenAlex.
- Dynamic Remodeling of the Zona Pellucida: Implications for Oocyte Competence and Assisted Reproduction.International journal of molecular sciences · 2025Review
- Article
- The Influence of Oviductal and Uterine Fluid Supplementation on the In Vitro Development and Quality of Cloned Sheep Embryos.Animals : an open access journal from MDPI · 2024Article
- Pre-fertilization approach using α-l-fucosidase modulates zona pellucida hardening during bovine in vitro embryo production.Veterinary research communications · 2024Article
- The role of oviduct-specific glycoprotein (OVGP1) in modulating biological functions of gametes and embryos.Histochemistry and cell biology · 2022Review
- Dynamic Changes in the Proteome of Early Bovine Embryos DevelopedFrontiers in cell and developmental biology · 2022Article
- Evidence of haptoglobin in the porcine female genital tract during oestrous cycle and its effect on in vitro embryo production.Scientific reports · 2021Article
- Physicochemical and Functional Characterization of Female Reproductive Fluids: A Report of the First Two Infants Born Following Addition of Their Mother's Fluids to the Embryo Culture Media.Frontiers in physiology · 2021Article
- A Comparative View on the Oviductal Environment during the Periconception Period.Biomolecules · 2020Review
- Extracellular Vesicles, the Road toward the Improvement of ART Outcomes.Animals : an open access journal from MDPI · 2020Review
- Extracellular Vesicles and the Oviduct Function.International journal of molecular sciences · 2020Review
- Roles of steroid hormones in oviductal functionReproduction (Cambridge, England) · 2020Review
- Identification of 56 Proteins Involved in Embryo-Maternal Interactions in the Bovine Oviduct.International journal of molecular sciences · 2020Article
- Which Low-Abundance Proteins are Present in the Human Milieu of Gamete/Embryo Maternal Interaction?International journal of molecular sciences · 2019Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Previously, our group demonstrated that recombinant porcine oviductin (pOVGP1) binds to the zona pellucida (ZP) of in vitro-matured (IVM) porcine oocytes with a positive effect on in vitro fertilization (IVF). The fact that pOVGP1 was detected inside IVM oocytes suggested that this protein had a biological role during embryo development. The aim of this study was to evaluate the effects of pOVGP1 on bovine in vitro embryo development. We applied 10 or 50 µg/ml of pOVGP1 during IVF, embryonic in vitro culture (IVC), or both, to evaluate cleavage and embryo development. Blastocyst quality was assessed by analyzing the expression of important developmental genes and the survival rates after vitrification/warming. pOVGP1 was detected in the ZP, perivitelline space, and plasma membrane of blastocysts. No significant differences (P > 0.05) were found in cleavage or blastocyst yield when 10 or 50 µg/ml of pOVGP1 was used during IVF or IVC. However, when 50 µg/ml pOVGP1 was used during IVF + IVC, the number of blastocysts obtained was half that obtained with the control and 10 µg/ml pOVGP1 groups. The survival rates after vitrification/warming of expanded blastocysts cultured with pOVGP1 showed no significant differences between groups (P > 0.05). The use of pOVGP1 during IVF, IVC, or both, increased the relative abundance of mRNA of DSC2, ATF4, AQP3, and DNMT3A, the marker-genes of embryo quality. In conclusion, the use of pOVGP1 during bovine embryo in vitro culture does not affect embryo developmental rates but produces embryos of better quality in terms of the relative abundance of specific genes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.