ArticleRNA (New York, N.Y.)2018
Reconstitution of 30S ribosomal subunits in vitro using ribosome biogenesis factors.
Article in RNA (New York, N.Y.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 37 citations in OpenAlex.
- Resurrecting tRNAs of the Last Universal Common Ancestor (Commonote) Toward Rebuilding the Ancient Translation System.Journal of molecular evolution · 2026Article
- Single-step in vitro reconstitution of theeLife · 2026Article
- Comparison of Immunoprotective Efficacy of Six Antigenic Proteins ofPathogens (Basel, Switzerland) · 2026Article
- Evolutionary Flexibility of Ribosome Biogenesis in Bacteria.Molecular biology and evolution · 2025Article
- Cell-Free Gene Expression: Methods and Applications.Chemical reviews · 2025Review
- De novo assembly and single-molecule study of kinetochore-microtubule interactions.Frontiers in cell and developmental biology · 2025Article
- Discovering optimal kinetic pathways for self-assembly using automatic differentiation.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Uncovering the Important Genetic Factors for Growth during Cefotaxime-Gentamicin Combination Treatment inAntibiotics (Basel, Switzerland) · 2023Article
- Emerging Quantitative Biochemical, Structural, and Biophysical Methods for Studying Ribosome and Protein-RNA Complex Assembly.Biomolecules · 2023Review
- Yet Another Similarity between Mitochondrial and Bacterial Ribosomal Small Subunit Biogenesis Obtained by Structural Characterization of RbfA fromInternational journal of molecular sciences · 2023Article
- GTPase Era at the heart of ribosome assembly.Frontiers in molecular biosciences · 2023Review
- Reconstitution of Mammalian Mitochondrial Translation System Capable of Long Polypeptide Synthesis.Methods in molecular biology (Clifton, N.J.) · 2023Article
- In vitro reconstitution of the Escherichia coli 70S ribosome with a full set of recombinant ribosomal proteins.Journal of biochemistry · 2022Article
- Efficient and Precise Protein Synthesis in a Cell-Free System Using a Set of In Vitro Transcribed tRNAs with Nucleotide Modifications.Methods in molecular biology (Clifton, N.J.) · 2022Article
- The many faces of RNA-based RNase P, an RNA-world relic.Trends in biochemical sciences · 2021Review
- Protein cofactors and substrate influence Mg2+-dependent structural changes in the catalytic RNA of archaeal RNase P.Nucleic acids research · 2021Article
- One-carbon metabolism, folate, zinc and translation.Microbial biotechnology · 2020Article
- Autonomous synthesis and assembly of a ribosomal subunit on a chip.Science advances · 2020Article
- In vitro reconstitution of functional small ribosomal subunit assembly for comprehensive analysis of ribosomal elements in E. coli.Communications biology · 2020Article
- Mutational characterization and mapping of the 70S ribosome active site.Nucleic acids research · 2020Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Reconstitution of ribosomes in vitro from individual ribosomal proteins provides a powerful tool for understanding the ribosome assembly process including the sequential incorporation of ribosomal proteins. However, conventional assembly methods require high-salt conditions for efficient ribosome assembly. In this study, we reconstituted 30S ribosomal subunits from individually purified ribosomal proteins in the presence of ribosome biogenesis factors. In this system, two GTPases (Era and YjeQ) facilitated assembly of a 30S subunit exhibiting poly(U)-directed polyphenylalanine synthesis and native protein synthesis under physiological conditions. This in vitro system permits a study of the assembly process and function of ribosome biogenesis factors, and it will facilitate the generation of ribosomes from DNA without using cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.