Evidence map›Paper›PMID 30076195›Full record

ArticleApplied and environmental microbiology2018

Automated Microscopic Analysis of Metal Sulfide Colonization by Acidophilic Microorganisms.

Sören Bellenberg, Antoine Buetti-Dinh, Vanni Galli, Olga Ilie, Malte Herold, Stephan Christel, Mariia Boretska, Igor V Pivkin, Paul Wilmes, Wolfgang Sand and 2 more

Erratum issuedOpen access · bronzeAbstract read
In one paragraph

Article in Applied and environmental microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
1.2field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 23 citations in OpenAlex.

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  12. Weak Iron Oxidation byFrontiers in microbiology · 2018
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

12 authors at 7 institutions in 6 countries.

Sören Bellenberg *Fakultät für Chemie, Biofilm Centre, Universität Duisburg-Essen, Essen, Germany soeren.bellenberg@uni-due.de antoine.buetti.dinh@usi.ch.
Antoine Buetti-Dinh *Institute of Computational Science, Faculty of Informatics, Università della Svizzera Italiana, Lugano, Switzerland soeren.bellenberg@uni-due.de antoine.buetti.dinh@usi.ch.ORCID 0000-0002-6469-0296
Vanni GalliInstitute for Information Systems and Networking, University of Applied Sciences of Southern Switzerland, Manno, Switzerland.
Olga IlieInstitute of Computational Science, Faculty of Informatics, Università della Svizzera Italiana, Lugano, Switzerland.
Malte HeroldLuxembourg Centre for Systems Biomedicine, University of Luxembourg, Belvaux, Luxembourg.
Stephan ChristelCentre for Ecology and Evolution in Microbial Model Systems, Linnaeus University, Kalmar, Sweden.ORCID 0000-0003-0021-2452
Mariia BoretskaFakultät für Chemie, Biofilm Centre, Universität Duisburg-Essen, Essen, Germany.
Igor V PivkinInstitute of Computational Science, Faculty of Informatics, Università della Svizzera Italiana, Lugano, Switzerland.
Paul WilmesLuxembourg Centre for Systems Biomedicine, University of Luxembourg, Belvaux, Luxembourg.
Wolfgang SandFakultät für Chemie, Biofilm Centre, Universität Duisburg-Essen, Essen, Germany.
Mario VeraInstitute for Biological and Medical Engineering, Schools of Engineering, Medicine & Biological Sciences, Department of Hydraulic & Environmental Engineering, Pontificia Universidad Católica de Chile, Santiago, Chile.
Mark DopsonCentre for Ecology and Evolution in Microbial Model Systems, Linnaeus University, Kalmar, Sweden.ORCID 0000-0002-9622-3318
SIB Swiss Institute of Bioinformatics · CHLinnaeus University · SEUniversity of Duisburg-Essen · DEUniversity of Luxembourg · LUDonghua University · CNPontificia Universidad Católica de Chile · CLUniversity of Applied Sciences and Arts of Southern Switzerland · CH

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Industrial biomining processes are currently focused on metal sulfides and their dissolution, which is catalyzed by acidophilic iron(II)- and/or sulfur-oxidizing microorganisms. Cell attachment on metal sulfides is important for this process. Biofilm formation is necessary for seeding and persistence of the active microbial community in industrial biomining heaps and tank reactors, and it enhances metal release. In this study, we used a method for direct quantification of the mineral-attached cell population on pyrite or chalcopyrite particles in bioleaching experiments by coupling high-throughput, automated epifluorescence microscopy imaging of mineral particles with algorithms for image analysis and cell quantification, thus avoiding human bias in cell counting. The method was validated by quantifying cell attachment on pyrite and chalcopyrite surfaces with axenic cultures of

Indexed as

Bacterial AdhesionAcidithiobacillusAlgorithmsAutomation, LaboratoryBacteriaBiofilmsCopperIronMetalsMicrobial ConsortiaMicroscopySulfidesSulfurchalcopyriteCopperIronMetalspyriteSulfidesSulfurbiofilm dispersalbiofilm formationbiofilmsbioleachingdiffusible soluble factorfluorescent image analysisimage analysismicrobe-mineral interactionmicrobe-mineral interactionsquorum sensing

Identifiers

PMID30076195
PMCPMC6182891
OpenAlexW2887021290

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.