Evidence map›Paper›PMID 30075851›Full record

ArticleBiophysical journal2018

Spatiomechanical Modulation of EphB4-Ephrin-B2 Signaling in Neural Stem Cell Differentiation.

Meimei Dong, Dawn P Spelke, Young Kwang Lee, Jean K Chung, Cheng-Han Yu, David V Schaffer, Jay T Groves

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biophysical journal, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
1.2field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 17 citations in OpenAlex.

  1. Trial
  2. Article
  3. Eph receptor signaling complexes in the plasma membrane.Trends in biochemical sciences · 2024
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  6. Article
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 2 countries.

Meimei DongDepartment of Chemistry, University of California Berkeley, Berkeley, California; Biophysics Graduate Group, University of California Berkeley, Berkeley, California.
Dawn P SpelkeDepartment of Chemical Engineering, University of California Berkeley, Berkeley, California; Department of Bioengineering, University of California Berkeley, Berkeley, California.
Young Kwang LeeDepartment of Chemistry, University of California Berkeley, Berkeley, California.
Jean K ChungDepartment of Chemistry, University of California Berkeley, Berkeley, California.
Cheng-Han YuMechanobiology Institute, National University of Singapore, Singapore, Singapore.
David V SchafferDepartment of Chemical Engineering, University of California Berkeley, Berkeley, California; Department of Bioengineering, University of California Berkeley, Berkeley, California. Electronic address: schaffer@berkeley.edu.
Jay T GrovesDepartment of Chemistry, University of California Berkeley, Berkeley, California; Biophysics Graduate Group, University of California Berkeley, Berkeley, California. Electronic address: jtgroves@lbl.gov.
University of California, Berkeley · USNational University of Singapore · SG

Funding

Understand the metabolic fitness of naïve T cellsP01AI091580 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI ARTHUR WEISS · 2011 to 2026
$31.1M
ECM geometrical and mechanical properties modulate RTK signalingU01CA202241 · NCI · UNIVERSITY OF CALIFORNIA BERKELEY · PI GROVES, JAY T., WEAVER, VALERIE MARIE · 2015 to 2019
$3.3M
Multivalent Ligands to Control Stem Cell FateR01NS083856 · NINDS · UNIVERSITY OF CALIFORNIA BERKELEY · PI KANE, RAVI S., SCHAFFER, DAVID V · 2014 to 2018
$1.7M
NCI NIH HHS U01 CA202241NINDS NIH HHS R01 NS083856
6 · The paper itself

Abstract

Interactions between EphB4 receptor tyrosine kinases and their membrane-bound ephrin-B2 ligands on apposed cells play a regulatory role in neural stem cell differentiation. With both receptor and ligand constrained to move within the membranes of their respective cells, this signaling system inevitably experiences spatial confinement and mechanical forces in conjunction with receptor-ligand binding. In this study, we reconstitute the EphB4-ephrin-B2 juxtacrine signaling geometry using a supported-lipid-bilayer system presenting laterally mobile and monomeric ephrin-B2 ligands to live neural stem cells. This experimental platform successfully reconstitutes EphB4-ephrin-B2 binding, lateral clustering, downstream signaling activation, and neuronal differentiation, all in a configuration that preserves the spatiomechanical aspects of the natural juxtacrine signaling geometry. Additionally, the supported bilayer system allows control of lateral movement and clustering of the receptor-ligand complexes through patterns of physical barriers to lateral diffusion fabricated onto the underlying substrate. The results from this study reveal a distinct spatiomechanical effect on the ability of EphB4-ephrin-B2 signaling to induce neuronal differentiation. These observations parallel similar studies of the EphA2-ephrin-A1 system in a very different biological context, suggesting that such spatiomechanical regulation may be a common feature of Eph-ephrin signaling.

Indexed as

Cell DifferentiationMechanical PhenomenaSignal TransductionAnimalsBiomechanical PhenomenaCell MembraneEphrin-B2MiceNeural Stem CellsReceptor, EphB4Ephrin-B2Receptor, EphB4

Identifiers

PMID30075851
PMCPMC6127455
OpenAlexW2883402309

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.