Evidence map›Paper›PMID 30075778›Full record

ArticleBMC biology2018

Structural and functional characterization of protein-lipid interactions of the Salmonella typhimurium melibiose transporter MelB.

Parameswaran Hariharan, Elena Tikhonova, João Medeiros-Silva, Aike Jeucken, Mikhail V Bogdanov, William Dowhan, Jos F Brouwers, Markus Weingarth, Lan Guan

Open access · goldAbstract read
In one paragraph

Article in BMC biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.

0numbers the graph read from it
0cells of the map it votes in
23citing papers in PubMed
1.6field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

23 citing papers in PubMed, 43 citations in OpenAlex.

  1. Article
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  5. Review
  6. Mobile barrier mechanisms for NabioRxiv : the preprint server for biology · 2023
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  11. Specific Lipid Studies in Complex Membranes by Solid-State NMR Spectroscopy.Chemistry (Weinheim an der Bergstrasse, Germany) · 2022
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 2 countries.

Parameswaran HariharanDepartment of Cell Physiology and Molecular Biophysics, Center for Membrane Protein Research, School of Medicine, Texas Tech University Health Sciences Center, Lubbock, TX, 79430, USA.
Elena TikhonovaDepartment of Cell Physiology and Molecular Biophysics, Center for Membrane Protein Research, School of Medicine, Texas Tech University Health Sciences Center, Lubbock, TX, 79430, USA.
João Medeiros-SilvaNMR Spectroscopy, Bijvoet Center for Biomolecular Research, Department of Chemistry, Faculty of Science, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands.
Aike JeuckenDepartment of Biochemistry & Cell Biology, Lipidomics Facility, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 2, 3584 CM, Utrecht, The Netherlands.
Mikhail V BogdanovDepartment of Biochemistry and Molecular Biology, the University of Texas Health Science, Center McGovern Medical School, Houston, TX, 77030, USA.
William DowhanDepartment of Biochemistry and Molecular Biology, the University of Texas Health Science, Center McGovern Medical School, Houston, TX, 77030, USA.
Jos F BrouwersDepartment of Biochemistry & Cell Biology, Lipidomics Facility, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 2, 3584 CM, Utrecht, The Netherlands.
Markus WeingarthNMR Spectroscopy, Bijvoet Center for Biomolecular Research, Department of Chemistry, Faculty of Science, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands. M.H.Weingarth@uu.nl.
Lan GuanDepartment of Cell Physiology and Molecular Biophysics, Center for Membrane Protein Research, School of Medicine, Texas Tech University Health Sciences Center, Lubbock, TX, 79430, USA. Lan.Guan@ttuhsc.edu.ORCID 0000-0002-2274-361X
Utrecht University · NLTexas Tech University · USThe University of Texas Health Science Center at Houston · US

Funding

Protein sequence determinants and properties of the lipid bilayer that govern membrane protein dynamic organizationR01GM121493 · NIGMS · UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON · PI BOGDANOV, MIKHAIL V., DOWHAN, WILLIAM · 2017 to 2025
$3.6M
Integrated approaches to symport mechanisms of membrane transportersR01GM122759 · NIGMS · TEXAS TECH UNIVERSITY HEALTH SCIS CENTER · PI GUAN, LAN · 2018 to 2021
$1.4M
Recombinant expression and functional characterization of a lysophosphatidylcholine transporterR21NS105863 · NINDS · TEXAS TECH UNIVERSITY HEALTH SCIS CENTER · PI GUAN, LAN · 2018 to 2019
$421k
NIGMS NIH HHS R01 GM121493NIGMS NIH HHS R01 GM122759NIGMS NIH HHS RO1GM121493NINDS NIH HHS R21NS105863
6 · The paper itself

Abstract

backgroundMembrane lipids play critical roles in the structure and function of membrane-embedded transporters. Salmonella typhimurium MelB (MelB

resultsLipidomic analysis and thin-layer chromatography (TLC) experiments reveal that at least one phosphatidylethanolamine (PE) and one phosphatidylglycerol (PG) molecule associate with MelB

conclusions(1) Lipid tails and glycerol backbones of interacting PE and PG may contribute to the stability of the structure of MelB

Indexed as

Bacterial ProteinsCardiolipinsMelibiosePhosphatidylethanolaminesPhosphatidylglycerolsSalmonella typhimuriumSymportersBacterial ProteinsCardiolipinsMelibiosemelibiose permeasephosphatidylethanolaminePhosphatidylethanolaminesPhosphatidylglycerolsSymportersCircular dichroism spectroscopyMass spectrometryMelting temperatureMembrane proteinPhospholipidsSolid-state NMRSubstrate bindingSugar transport

Identifiers

PMID30075778
PMCPMC6091025
OpenAlexW2886347967

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.