ArticleParasitology research2018
Diagnostic comparison of Baermann funnel, Koga agar plate culture and polymerase chain reaction for detection of human Strongyloides stercoralis infection in Maluku, Indonesia.
Article in Parasitology research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT02105714 (Diagnosis of Neglected Tropical Diseases), which is not on this map. Cited by 18 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Diagnosis of Neglected Tropical Diseases (NTDs) in Patients Presenting With Persistent Digestive Disorders (≥2 Weeks) in Côte d'Ivoire, Indonesia, Mali and Nepal
Who cites it
18 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Diagnostic accuracy of nucleic acid amplification tests for human intestinal nematode infections: A systematic review and meta-analysis.PLoS neglected tropical diseases · 2026Pooled it
- Systematic Review of Strongyloides stercoralis Infection Diagnosis in Southeast Asia: Insights from Parasitological, Molecular, and Serological Approaches.The American journal of tropical medicine and hygiene · 2024Pooled it
- Circulating Leukocytes and Antibody Isotype Reactivity in Rats Experimentally Infected with Different Burdens ofTropical medicine and infectious disease · 2026Article
- The Recombinase Polymerase Amplification Test forPathogens (Basel, Switzerland) · 2024Article
- Modifications to the parasitological technique of Rugai increase the diagnostic sensitivity for strongyloidiasis.Parasitology research · 2024Article
- Review
- From past to present: opportunities and trends in the molecular detection and diagnosis of Strongyloides stercoralis.Parasites & vectors · 2023Review
- Improved diagnostic sensitivity of human strongyloidiasis using point-of-care mixed recombinant antigen-based immunochromatography.Parasite (Paris, France) · 2023Article
- Strongyloides stercoralis infection in dogs in Austria: two case reports.Parasites & vectors · 2022Article
- Development and Efficacy of Droplet Digital PCR for Detection of Strongyloides stercoralis in Stool.The American journal of tropical medicine and hygiene · 2021Article
- Performance evaluation of Baermann techniques: The quest for developing a microscopy reference standard for the diagnosis of Strongyloides stercoralis.PLoS neglected tropical diseases · 2021Article
- Direct detection of Strongyloides infection via molecular and antigen detection methods.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2021Review
- Effectiveness ofDiagnostics (Basel, Switzerland) · 2020Article
- Epidemiology of intestinal helminthiases in a rural community of Ethiopia: Is it time to expand control programs to include Strongyloides stercoralis and the entire community?PLoS neglected tropical diseases · 2020Article
- Article
- An optimized agar plate culture improves diagnostic efficiency for Strongyloides stercoralis infection in an endemic community.Parasitology research · 2020Article
- Development of immunochromatographic device as a point-of-care tool for serodiagnosis of human strongyloidiasis cases.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2020Article
- [Acute renal failure and rare severe complication of systemic steroid treatment in a 73-year-old woman].Der Internist · 2019Review
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
Human infection with the nematode Strongyloides stercoralis, which may have a life-threatening course, primarily occurs in tropical settings. Epidemiological data on the occurrence of strongyloidiasis are scarce, and microscopic stool-based detection methods are insensitive. Polymerase chain reaction (PCR) assays have been developed, yet conflicting results have been reported. Our goal was to determine whether there was diagnostic agreement between an in-house PCR and two microscopic techniques, the Baermann funnel (BM) and the Koga agar plate culture (KAP) for the detection of S. stercoralis in stool samples. Eighty ethanol-fixed stool samples stemming from a cross-sectional survey in Maluku, Indonesia, were purposefully selected for PCR analysis. The final sample size comprised four groups, each with 20 samples: group 1, positive for S. stercoralis on both BM and KAP; group 2, positive only by BM; group 3, positive only by KAP; and group 4, negative on both BM and KAP. A Strongyloides-specific PCR targeting the internal transcribed spacer 2 (ITS2) region was carried out in an Indonesian reference laboratory. The overall agreement between PCR and microscopy was 61% (49/80 samples), being highest in group 1 (15/20, 75%) and lowest in group 3 (9/20, 45%). PCR revealed eight additional S. stercoralis infections in group 4. Future studies should elucidate the 'true' infection status of samples that are negative by PCR, but positive upon microscopy. Taken together, there is a lack of agreement between microscopy and PCR results for the diagnosis of human S. stercoralis infection in Indonesia. ClinicalTrials.gov (identifier: NCT02105714).
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Registered trials
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