ArticleAnalytical chemistry2018
Electrochemical Measurements of Acetylcholine-Stimulated Dopamine Release in Adult Drosophila melanogaster Brains.
Article in Analytical chemistry, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 31 citations in OpenAlex.
- Advances in Targeting Central Cholinergic Dysfunction for Neurodegenerative Diseases: From Pharmacotherapy to Neuromodulation.CNS neuroscience & therapeutics · 2026Review
- Motor neurons integrate cholinergic inputs through spatial organization of diverse nicotinic receptors.PNAS nexus · 2026Article
- Transcriptomic investigation of agonistic behaviors of boxer shrimps (Stenopus species): insights into the potential neural signaling roles of dopamine and acetylcholine.BMC genomics · 2026Article
- Machine Learning for Separating Dopamine and Octopamine Electrochemical Signals in Drosophila.Analytical chemistry · 2026Article
- Article
- Fast-Scan Cyclic Voltammetry (FSCV) Reveals Behaviorally Evoked Dopamine Release by Sugar Feeding in the Adult Drosophila Mushroom Body.Angewandte Chemie (International ed. in English) · 2022Article
- Nano- and Microsensors for In Vivo Real-Time Electrochemical Analysis: Present and Future Perspectives.Nanomaterials (Basel, Switzerland) · 2022Review
- SSRI antidepressants differentially modulate serotonin reuptake and release in Drosophila.Journal of neurochemistry · 2022Article
- A genetically encoded sensor for measuring serotonin dynamics.Nature neuroscience · 2021Article
- In Silico Studies of Lamiaceae Diterpenes with Bioinsecticide Potential againstMolecules (Basel, Switzerland) · 2021Article
- Ring Finger Protein 11 (RNF11) Modulates Dopamine Release in Drosophila.Neuroscience · 2021Article
- Real-Time Measurement of Stimulated Dopamine Release in Compartments of the AdultAnalytical chemistry · 2020Article
- 3D-Printed Carbon Nanoelectrodes for In Vivo Neurotransmitter Sensing.Nano letters · 2020Article
- Measurement of natural variation of neurotransmitter tissue content in red harvester ant brains among different colonies.Analytical and bioanalytical chemistry · 2020Article
- Fundamentals of fast-scan cyclic voltammetry for dopamine detection.The Analyst · 2020Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The fruit fly, Drosophila melanogaster, is a popular model organism for studying neurological processes and diseases due to the availability of sophisticated genetic tools. While endogenous neurotransmitter release has been characterized in Drosophila larvae, here, we measured endogenous dopamine release in isolated adult Drosophila brains for the first time. Dopamine was measured with fast-scan cyclic voltammetry (FSCV), and acetylcholine or nicotine were used as the stimulus, as both interact with nicotinic acetylcholine receptors (nAChRs) to evoke endogenous dopamine release. Stimulations with 10 pmol of acetylcholine elicited 0.26 ± 0.05 μM dopamine, while 70 fmol nicotine stimulations evoked 0.29 ± 0.03 μM in the central complex. Nicotine-stimulated dopamine release lasted much longer than acetylcholine-stimulated release. Dopamine release is reduced in the presence of nAChR antagonist α-bungarotoxin and the sodium channel blocker tetrodotoxin, indicating release is mediated by nAChRs and exocytosis. The identity of dopamine was confirmed by using 3-iodotyrosine, a dopamine synthesis inhibitor, and by confirming that release was not changed in octopamine synthesis mutant flies, Tdc2
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.