Evidence map›Paper›PMID 30073492›Full record

ArticleJournal of biomolecular NMR2018

Improving yields of deuterated, methyl labeled protein by growing in H

Evan S O'Brien, Danny W Lin, Brian Fuglestad, Matthew A Stetz, Travis Gosse, Cecilia Tommos, A Joshua Wand

Open access · greenAbstract read
In one paragraph

Article in Journal of biomolecular NMR, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
1.8field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 24 citations in OpenAlex.

  1. Article
  2. Article
  3. Protein deuterationChemical communications (Cambridge, England) · 2024
    Article
  4. Article
  5. Cell-free synthesis of proteins with selectivelyMagnetic resonance (Gottingen, Germany) · 2023
    Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Alpha protons as NMR probes in deuterated proteins.Journal of biomolecular NMR · 2019
    Article
  11. Article
  12. Article
  13. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Evan S O'BrienJohnson Research Foundation and Department of Biochemistry & Biophysics, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, 19104-6059, USA.
Danny W LinJohnson Research Foundation and Department of Biochemistry & Biophysics, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, 19104-6059, USA.
Brian FuglestadJohnson Research Foundation and Department of Biochemistry & Biophysics, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, 19104-6059, USA.
Matthew A StetzJohnson Research Foundation and Department of Biochemistry & Biophysics, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, 19104-6059, USA.
Travis GosseJohnson Research Foundation and Department of Biochemistry & Biophysics, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, 19104-6059, USA.
Cecilia TommosJohnson Research Foundation and Department of Biochemistry & Biophysics, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, 19104-6059, USA.
A Joshua WandJohnson Research Foundation and Department of Biochemistry & Biophysics, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, 19104-6059, USA. wand@mail.med.upenn.edu.ORCID http://orcid.org/0000-0001-8341-0782
Johnson Foundation · US

Funding

STRUCTURAL BIOLOGY TRAINING PROGRAMT32GM008275 · NIGMS · UNIVERSITY OF PENNSYLVANIA · PI WAND, A. JOSHUA · 1988 to 2018
$6.0M
Understanding Protein RadicalsR01GM079190 · NIGMS · UNIVERSITY OF PENNSYLVANIA · PI TOMMOS, CECILIA · 2008 to 2024
$3.6M
National Institute of General Medical Sciences R01 GM079190National Institute of General Medical Sciences T32 GM008275NIGMS NIH HHS R01 GM079190NIGMS NIH HHS T32 GM008275
6 · The paper itself

Abstract

Solution NMR continues to make strides in addressing protein systems of significant size and complexity. A fundamental requirement to fully exploit the

Indexed as

Aldo-Keto Reductase Family 1 Member C3Amino AcidsDeuteriumFlavodoxinHumansIsotope LabelingNuclear Magnetic Resonance, BiomolecularSensory RhodopsinsStramenopilesUbiquitinAKR1C3 protein, humanAldo-Keto Reductase Family 1 Member C3Amino AcidsDeuteriumFlavodoxinSensory RhodopsinsUbiquitinAlgal amino acidsAmino acid chromatographyILVM methyl labelingNuclear magnetic resonanceProtein deuteration

Identifiers

PMID30073492
PMCPMC6165672
OpenAlexW2886727733

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.